Genetic factors and diet affect long-bone length in the F34 LG,SM advanced intercross.
Genetic factors and diet affect long-bone length in the F34 LG,SM advanced intercross.
复制标题
遗传因素和饮食影响 F34 LG、SM 高级杂交中的长骨长度。
DOI：
10.1007/s00335-010-9311-5
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Cheverud,JamesM
中科院分区：
文献类型：
--
作者：
Norgard,ElizabethA;Lawson,HeatherA;Pletscher,LSusan;Wang,Bing;Brooks,VictoriaR;Wolf,JasonB;Cheverud,JamesM
先前对LG、SM高级杂交系的研究已经确定了大约40个长骨（肱骨、尺骨、股骨和胫骨）长度的数量性状位点（QTL）。本研究以LG、SM高级杂交F34代（n= 1424）为材料，对长骨长度QTL进行了精细定位。通过将人群按性别分为高脂肪和低脂肪饮食，产生8个性别/饮食队列，评估环境影响。我们鉴定了145个骨长QTL，其中45个为多效性QTL; 69个重复QTL来自先前的研究，35个是在先前鉴定的位点显著的新性状，41个是新QTL。许多QTL仅影响基于性别和/或饮食的群体的子集。10个已知的骨骼生长基因中有8个在3周龄的LG/J男性胫骨近端生长板相对于SM/J上调。亲本菌株LG/J和SM/J的序列表明，在这45个QTL的置信区间中存在超过50万个多态性。我们检测了526个多态性，发现97个代表氨基酸组成的根本变化，而40个被预测为对蛋白质功能有害，需要额外的实验来了解基因调控或蛋白质功能的变化如何改变遗传结构并与环境相互作用产生表型变异。
Previous studies on the LG,SM advanced intercross line have identified approximately 40 quantitative trait loci (QTL) for long -bone (humerus, ulna, femur, and tibia) lengths. In this study, long-bone-length QTL were fine-mapped in the F34generation (n= 1424) of the LG,SM advanced intercross. Environmental effects were assessed by dividing the population by sex between high-fat and low-fat diets, producing eight sex/diet cohorts. We identified 145 individual bone-length QTL comprising 45 pleiotropic QTL; 69 replicated QTL from previous studies, 35 were new traits significant at previously identified loci, and 41 were novel QTL. Many QTL affected only a subset of the population based on sex and/or diet. Eight of ten known skeletal growth genes were upregulated in 3-week-old LG/J male proximal tibial growth plates relative to SM/J.The sequences of parental strains LG/J and SM/J indicated the presence of over half a million polymorphisms in the confidence intervals of these 45 QTL. We examined 526 polymorphisms and found that 97 represented radical changes to amino acid composition while 40 were predicted to be deleterious to protein function.Additional experimentation is required to understand how changes in gene regulation or protein function can alter the genetic architecture and interact with the environment to produce phenotypic variation.