Myogenetic oligodeoxynucleotide complexed with berberine promotes differentiation of chicken myoblasts
Myogenetic oligodeoxynucleotide complexed with berberine promotes differentiation of chicken myoblasts
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DOI：
10.1111/asj.13597
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发表时间：
2021-12-01
影响因子：
2
通讯作者：
Takaya, Tomohide
中科院分区：
文献类型：
--
作者：
Nihashi, Yuma;Shinji, Sayaka;Takaya, Tomohide
成肌细胞是在肌肉形成过程中发育成肌管的肌源性前体。提高成肌细胞的分化效率对于提高家畜的产肉率具有重要意义。我们最近发现了新的寡脱氧核苷酸（ODNs）称为肌源性ODNs（myoDNs），促进哺乳动物成肌细胞的分化。异喹啉生物碱小檗碱与其中一种myoDN iSN 04形成复合物，并增强其活性。本研究探讨myoDNs对鸡成肌细胞的影响，以阐明其物种特异性作用。发现七种myoDNs（iSN 01-iSN 07）促进鸡成肌细胞分化为肌球蛋白重链（MHC）阳性肌管。iSN 04-黄连素复合物表现出比单独iSN 04更高的成肌活性，其显示出增强成肌细胞分化成肌管和成肌基因表达（MyoD、肌细胞生成素、MHC和肌细胞因子）的上调。这些数据表明，促进鸡成肌细胞分化的myoDNs可用作肉鸡日粮中潜在的饲料添加剂。
Myoblasts are myogenic precursors that develop into myotubes during muscle formation. Improving efficiency of myoblast differentiation is important for advancing meat production by domestic animals. We recently identified novel oligodeoxynucleotides (ODNs) termed myogenetic ODNs (myoDNs) that promote the differentiation of mammalian myoblasts. An isoquinoline alkaloid, berberine, forms a complex with one of the myoDNs, iSN04, and enhances its activities. This study investigated the effects of myoDNs on chicken myoblasts to elucidate their species-specific actions. Seven myoDNs (iSN01-iSN07) were found to facilitate the differentiation of chicken myoblasts into myosin heavy chain (MHC)-positive myotubes. The iSN04-berberine complex exhibited a higher myogenetic activity than iSN04 alone, which was shown to enhance the differentiation of myoblasts into myotubes and the upregulation of myogenic gene expression (MyoD, myogenin, MHC, and myomaker). These data indicate that myoDNs promoting chicken myoblast differentiation may be used as potential feed additives in broiler diets.