A theory of centriole duplication based on self-organized spatial pattern formation
A theory of centriole duplication based on self-organized spatial pattern formation
复制标题
基于自组织空间格局形成的中心粒复制理论
DOI：
10.1083/jcb.201904156
复制
发表时间：
2019
影响因子：
7.8
通讯作者：
Kitagawa Daiju
中科院分区：
文献类型：
--
作者：
Takao Daisuke;Yamamoto Shohei;Kitagawa Daiju
在每个细胞周期中，中心粒被复制以产生每个预先存在的中心粒的单个拷贝。在中心粒复制开始时，主调节器Polo样激酶4（Plk4）在其围绕预先存在的中心粒的空间模式中经历动态变化，形成单个复制位点。然而，这种模式转变的意义和机制仍然未知。使用超分辨率成像，我们发现，中心粒Plk4表现出周期性的离散模式类似珍珠项链，经常与单一的突出病灶。数学建模和模拟结合Plk4的自组织特性成功地产生了实验观察到的模式。因此，我们认为Plk 4的自我模式化对于调节中心粒复制至关重要。这些结果，定义了自组织调节的机制，为理解中心粒重复提供了一个基本原则。
In each cell cycle, centrioles are duplicated to produce a single copy of each preexisting centriole. At the onset of centriole duplication, the master regulator Polo-like kinase 4 (Plk4) undergoes a dynamic change in its spatial pattern around the preexisting centriole, forming a single duplication site. However, the significance and mechanisms of this pattern transition remain unknown. Using super-resolution imaging, we found that centriolar Plk4 exhibits periodic discrete patterns resembling pearl necklaces, frequently with single prominent foci. Mathematical modeling and simulations incorporating the self-organization properties of Plk4 successfully generated the experimentally observed patterns. We therefore propose that the self-patterning of Plk4 is crucial for the regulation of centriole duplication. These results, defining the mechanisms of self-organized regulation, provide a fundamental principle for understanding centriole duplication.