LOW-MOLECULAR-WEIGHT FACTOR-X ACTIVATOR FROM CERASTES-VIPERA (SAHARA SAND VIPER) VENOM
LOW-MOLECULAR-WEIGHT FACTOR-X ACTIVATOR FROM CERASTES-VIPERA (SAHARA SAND VIPER) VENOM
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DOI：
10.1016/0041-0101(93)90260-p
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发表时间：
1993-08-01
期刊：
影响因子：
2.8
通讯作者：
ELASMAR, MF
中科院分区：
文献类型：
--
作者：
FARID, T;NASSER, H;ELASMAR, MF
用SephadexG 100柱层析纯化的蝰蛇毒组分G，经DEAE-SephadexA 50柱层析后，得到了一个新的组分G。获得因子X激活剂。它有一个摩尔。重量比为12，500，等电点为4.4。缩短了正常血浆和缺乏因子V、VII、VIII、IX、XI和XIII的血浆的凝血时间，而对缺乏因子X和因子II的血浆无影响。它具有丝氨酸蛋白酶活性和最小的纤溶酶活性。PMSF、亮抑酶肽和碘乙酰胺对其丝氨酸蛋白酶活性有明显的抑制作用。聚抗蛇毒血清可中和激活剂的凝血活性。
Fraction G from Cerastes vipera venom previously purified on Sephadex G100 was refractionated on DEAE-Sephadex A50 column. A factor X activator was obtained. It had a mol. wt of 12,500 and an isoelectric point of 4.4. It shortened the plasma recalcification time of normal plasma, and plasmas deficient in factors V, VII, VIII, IX, XI and XIII, while it had no effect on plasma deficient in factor X or factor II. It had a serine protease activity and a minimal plasmin activity. PMSF, leupeptin and iodoacetamide exerted a pronounced inhibitory effect on its serine protease activity. Polyantivenin could neutralize the coagulant activity of the activator.