Polymorphisms and haplotypes of the myostatin gene associated with growth in juvenile red sea bream Pagrus major
Polymorphisms and haplotypes of the myostatin gene associated with growth in juvenile red sea bream Pagrus major
复制标题
与幼真真鲷生长相关的肌肉生长抑制素基因多态性和单倍型
DOI：
10.1111/are.14766
复制
发表时间：
2020
影响因子：
2
通讯作者：
Sawayama Eitaro
中科院分区：
文献类型：
--
作者：
Sawayama Eitaro
肌肉抑制素基因(MSTN)在包括几个水产养殖物种在内的脊椎动物中的多态性已经被研究，揭示了它们在生长中的作用。本研究试图找出与日本重要养殖鱼种--养殖罗非鱼幼鱼生长性状相关的MSTN基因多态性。MSTN基因编码区存在6个外显子多态性：外显子1缺失1个，外显子2缺失1个单核苷酸多态(SNP)，外显子3有4个SNPs。外显子1的缺失消除了聚谷氨酰胺结构区域的一个密码子(谷氨酰胺)，但所有SNP都是同义的。我们分析了在孵化后50天从商业生产中获得的大型和小型大型对虾标本的多态与生长表型的关系。位于外显子3的两个SNPs(c.+846T>；C和c.+1140T>；C)在大表型组中显著增加，而与小表型无显著关联。利用两个表型组的基因信息重建单倍型，成功构建了9个单倍型(Hap_1~Hap_9)。HAP_6和HAP_7分别在小群体和大群体中明显增多。对个体标本的双倍型(单倍型组合)进行分析，发现19个双倍型。Hap_7/Hap_7双倍型在大型组中占46.7%，显著高于小型组(13.5%)。这些结果将有助于分子标记辅助选择，以提高产量和生长。
Polymorphisms of the myostatin gene (MSTN) have been studied in vertebrates including several aquaculture species, revealing their role in growth. We attempted to identify polymorphisms in MSTN associated with growth traits of juvenile farmed red sea bream Pagrus major, an important cultured fish species in Japan. Polymorphisms in the coding region of MSTN were screened, and six polymorphisms were found among three exons: a deletion in exon 1, a single nucleotide polymorphism (SNP) in exon 2 and four SNPs in exon 3. The deletion in exon 1 eliminated a codon (glutamine) from the region comprising the polyglutamine structure, but all SNPs were synonymous. We analysed the polymorphisms for association with growth phenotype in large and small P. major specimens obtained from commercial production at 50 days post hatching. Two SNPs located in exon 3 (c.+846T>C and c.+1140T>C) were significantly more frequent in the large group, while no SNPs were significantly associated with the small phenotype. Haplotypes were reconstructed using genotype information of the two phenotype groups, and nine haplotypes (Hap_1 to Hap_9) were successfully reconstructed. Hap_6 and Hap_7 were significantly more often observed in small and large groups respectively. Analysis of diplotypes (haplotype combinations) of individual specimens revealed 19 diplotypes. The Hap_7/Hap_7 diplotype was found in 46.7% of large phenotype specimens, significantly more frequently than in the small group (13.5%). These results will be useful for marker‐assisted selection of red sea bream to improve production with respect to growth.