Unravelling the pathway of respiratory toxicity in goldlined seabream (Rhabdosargus sarba) induced by the harmful alga Chattonella marina.
Unravelling the pathway of respiratory toxicity in goldlined seabream (Rhabdosargus sarba) induced by the harmful alga Chattonella marina.
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DOI：
10.1016/j.aquatox.2011.04.014
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发表时间：
2011-08
影响因子：
4.5
通讯作者：
Min Shen;Jingliang Xu;M. Chiang;D. Au
中科院分区：
文献类型：
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作者：
Min Shen;Jingliang Xu;M. Chiang;D. Au
赤潮生物Chattonellamarina在世界范围内造成了大量的鱼类死亡和经济损失。窒息通常被认为是导致鱼类死亡的主要原因。码头然而，导致鱼类呼吸障碍和随后的鱼类死亡的具体机制还不清楚。marinaremain未知。对C. marina的呼吸功能相关生理、组织病理和生化指标的时间变化。码头暴露于C的金线鲷未发现血红蛋白氧化和溶血。海水的存在可能不是导致应激鱼pO 2下降的主要原因。鳃的组织病理学特征为鳃组织不规则，鳃间有粘液和藻细胞。暴露在海水中的鱼在暴露于C后不久，金线鲷的血浆乳酸出现激增。marina（0.5 h），并在整个暴露期间持续，表明C.暴露在海水中的鱼血浆葡萄糖的消耗在显示应激症状和濒临死亡的金线鲷中是明显的。然而，鱼活在C。marinabloom没有表现出血浆葡萄糖消耗。结果表明，可发酵燃料的可用性所示的葡萄糖水平是至关重要的，以确定鱼类生存在C。marina暴露。总的来说，我们的研究结果驳斥了溶血素和/或一氧化氮是C的罪魁祸首。对鱼类的毒性。本研究首次阐明了有害藻C的呼吸毒性途径。腌鱼。
The harmful algaChattonellamarinahas caused massive fish kills and economic losses worldwide. Suffocation is generally believed to be the major cause of fish death byC. marina. However, the specific mechanisms leading to respiratory disorder in fish and subsequent fish kills byC. marinaremain unknown. The goldlined seabream, highly susceptible toC. marina, was employed to investigate temporal changes of physiological, histopathological and biochemical parameters related to respiratory function at different stages of exposure toC. marina. Hemoglobin oxidation and blood lyses were not found in goldlined seabream exposed toC. marina, which could not be the key reasons accounting for pO2drop in the stressed fish. Gill histopathology such as irregular organization of lamellae, mucous with algal cells trapped in interfilamental spaces, were typical inC. marinaexposed fish. A surge of plasma lactate occurred in goldlined seabream shortly after exposure toC. marina(0.5 h) and sustained throughout the exposure period, indicating rapid onset of and persistent anaerobic respiration inC. marinaexposed fish. Depletion of plasma glucose was clearly evident in goldlined seabream showing stress symptoms and near death. Yet, fish alive in theC. marinabloom did not exhibit plasma glucose depletion. The results suggest that availability of fermentable fuel as indicated by glucose level is critical to determine fish survival inC. marinaexposure. Overall, our findings have rebuked the involvement of hemolysins and/or nitric oxide as the culprits forC. marinatoxicity to fish. This study is the first to demonstrate the pathway of respiratory toxicity induced by the harmful algaC. marinain fish.