Multiple combinations of alternatively spliced exons in rat tropomyosin-α gene mRNA:: Evidence for 20 new isoforms in adult tissues and cultured cells
Multiple combinations of alternatively spliced exons in rat tropomyosin-α gene mRNA:: Evidence for 20 new isoforms in adult tissues and cultured cells
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DOI：
10.1006/abbi.2001.2347
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发表时间：
2001-06-01
影响因子：
3.9
通讯作者：
Bergtrom, G
中科院分区：
文献类型：
--
作者：
Cooley, BC;Bergtrom, G
先前使用Northern印迹和核糖核酸酶保护测定方法对原肌球蛋白-a基因的研究鉴定了通过外显子选择性剪接产生的九种亚型的表达。其中一些亚型被表征为组织特异性和/或发育特异性。本研究使用高度灵敏的基于 RT-PCR 的策略来测定这些以及许多新亚型在各种成年大鼠组织中的表达。发现 Ah 9 亚型在所有评估的组织中都有表达。此外，还鉴定出了 20 种具有不同组织特异性的新亚型。序列分析证实了外显子剪接模式。这种更大程度的同工型生成与另一个原肌球蛋白基因TM-5基因的最新发现相似，还显示了新的同工型的产生，特别是使用羧基末端编码外显子的新连接的同工型。几种新的基于cDNA的同工型预测原肌球蛋白种类比先前表征的高分子量原肌球蛋白-cu基因同工型长10个氨基酸。原肌球蛋白亚型的表达明显缺乏显着的组织特异性，这表明许多这些亚型在细胞功能中具有更通用的作用。 (C) 2001 年学术出版社。
Previous studies of the tropomyosin-a gene using Northern blot and ribonuclease protection assay methods identified the expression of nine isoforms generated by alternative splicing of exons, Several of these isoforms were characterized as tissue-specific and/or developmentally specific. The present study used a highly sensitive RT-PCR-based strategy to assay the expression of these and many novel isoforms in a variety of adult rat tissues. Ah 9 isoforms were found to be expressed in all tissues evaluated. Furthermore, 20 new isoforms were identified with varying tissue specificity. Sequence analysis confirmed exon splicing patterns. This greater degree of isoform generation parallels recent findings for another tropomyosin gene, the TM-5 gene, for which the generation of new isoforms, in particular, ones using novel junctions for carboxy-terminal-coding exons, was also shown, Several of the new cDNA-based isoforms predict tropomyosin protein species that are 10 amino acids longer than previously characterized high-molecular-weight tropomyosin-cu gene isoforms. The apparent lack of significant tissue specificity in the expression of tropomyosin isoforms suggests that many of these isoforms have more generic roles in cell function. (C) 2001 Academic Press.