Presence of phosphatidylcholine hydroperoxide in human plasma.
Presence of phosphatidylcholine hydroperoxide in human plasma.
复制标题
人血浆中存在氢过氧化磷脂酰胆碱。
DOI：
--
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
T. Kaneda
中科院分区：
文献类型：
--
作者：
T. Miyazawa;K. Yasuda;K. Fujimoto;T. Kaneda
建立了一种新的荧光-高效液相色谱法（CL-HPLC）测定人血浆中过氧化氢磷脂酰胆碱（PCOH）的方法。该方法包括分离磷脂酰胆碱衍生物从血浆脂质正相高效液相色谱法和随后的检测过氧化氢依赖性化学发光（CL）的PCOH。CL是在过氧化氢和细胞色素c-血红素反应过程中通过鲁米诺氧化而产生的。对氢过氧化物的高特异性允许在50- 2，000 pmol氢过氧化物-O2的浓度范围内对大PCOOH范围进行灵敏测定。使用该方法，强烈建议并定量证实正常人血浆中存在PCOH。
A chemiluminescence-high performance liquid chromatography (CL-HPLC) system was newly developed and used for the hydroperoxide-specific determination of phosphatidylcholine hydroperoxide (PCOOH) in human plasma. The method involves separation of phosphatidylcholine derivatives from plasma lipids by normal phase HPLC and subsequent detection of hydroperoxide-dependent chemiluminescence (CL) of PCOOH. CL was produced through luminol oxidation during the reaction of the hydroperoxide and cytochrome c-heme. The high specificity for the hydroperoxide allows the sensitive assaying of a large PCOOH range over a concentration range of 50-2,000 pmol of hydroperoxide-O2. Using this method, the occurrence of PCOOH in normal human plasma was strongly suggested and was confirmed quantitatively.