CHARACTERIZATION AND DEVELOPMENT OF ROTATIONAL BEHAVIOR IN HELISOMA EMBRYOS - ROLE OF ENDOGENOUS SEROTONIN
CHARACTERIZATION AND DEVELOPMENT OF ROTATIONAL BEHAVIOR IN HELISOMA EMBRYOS - ROLE OF ENDOGENOUS SEROTONIN
复制标题
DOI：
10.1002/neu.480220905
复制
发表时间：
1991-12-01
期刊：
影响因子：
--
通讯作者：
GOLDBERG, JI
中科院分区：
文献类型：
--
作者：
DIEFENBACH, TJ;KOEHNCKE, NK;GOLDBERG, JI
池塘蜗牛 Helisoma trivolvis 胚胎所表现出的纤毛驱动的旋转行为的特征包括其行为子成分、发育变化和对外源性血清素的反应。人们发现旋转是一种复杂的行为，其特征在于四个参数：旋转方向、旋转速率、旋转波动和不活动周期。这些参数均表现出从胚胎阶段 E15 到阶段 E30 的特征性发育变化。值得注意的是，旋转速率和旋转喘振频率从阶段E15到E25增加，并在阶段E30下降到中间水平。总体轮换率的发育增加似乎主要是由浪涌频率的增加引起的，而不是非浪涌轮换率的增加。将胚胎浸入含有血清素的池塘水中会导致旋转速率呈剂量依赖性、可逆性增加，以及浪涌频率呈剂量依赖性、可逆性下降。血清素拮抗剂米安舍林消除了外源性血清素的兴奋作用。此外，单独使用米安色林可降低旋转速率并几乎消除旋转波动。总而言之，这些药理学结果表明内源性血清素是产生旋转激增的原因。鉴于早期胚胎在表达旋转激增的阶段仅包含一对血清素能神经元（Goldberg 和 Kater，1989），这些结果也提示了这样的假设：这些神经元（胚胎神经元 C1）在胚胎发育过程中充当纤毛兴奋性运动神经元。
Cilia-driven rotational behavior displayed by embryos of the pond snail Helisoma trivolvis was characterized in terms of its behavioral subcomponents, developmental changes, and response to exogenous serotonin. Rotation was found to be a complex behavior characterized by four parameters; rotational direction, rotation rate, rotational surges, and periods of inactivity. These parameters all exhibited characteristic developmental changes from embryonic stage E15 through stage E30. Notably, both rotation rate and frequency of rotational surges increased from stage E15 to E25 and declined to an intermediate level by stage E30. It appeared that the developmental increase in overall rotation rate was caused primarily by an increase in surge frequency, rather than an increase in the rate of nonsurge rotation. Immersion of embryos inserotonin-containing pond water resulted in a dose-dependent, reversible increase in rotation rate as well as a dose-dependent, reversible decrease in surge frequency. The serotonin antagonist, mianserin, abolished the excitatory effect of exogenous serotonin. Furthermore, application of mianserin alone reduced rotation rate and virtually abolished rotational surges. Taken together, these pharmacological results suggest that endogenous serotonin is responsible for generating rotational surges. Given that early embryos contain only a single pair of serotonergic neurons (Goldberg and Kater, 1989) during the stages when rotational surges are expressed, these results also prompt the hypothesis that these neurons, embryonic neurons C1, act as cilioexcitatory motor neurons during embryonic development.