DIFFERENTIAL-EFFECTS OF INTERLEUKIN-1-BETA AND INTERLEUKIN-2 ON GLIA AND HIPPOCAMPAL-NEURONS IN CULTURE
DIFFERENTIAL-EFFECTS OF INTERLEUKIN-1-BETA AND INTERLEUKIN-2 ON GLIA AND HIPPOCAMPAL-NEURONS IN CULTURE
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DOI：
10.1016/0736-5748(94)00072-b
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发表时间：
1995-06-01
影响因子：
1.8
通讯作者：
COTMAN, CW
中科院分区：
文献类型：
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作者：
ARAUJO, DM;COTMAN, CW
本研究旨在评估白细胞介素-1 β（IL-1 β）和白细胞介素-2（IL-2）对培养的神经胶质细胞和神经元细胞的影响。在从星形胶质细胞和小胶质细胞培养物收集的培养基中检测到IL-1 β样和IL-2样免疫反应性的存在，表明两种淋巴因子都可以从任一细胞类型释放。然而，在小胶质细胞介质中测得的水平显着高于星形胶质细胞介质。此外，培养基中IL-1 β样免疫反应性物质的含量约为IL-2的5 - 10倍，尽管小胶质细胞和星形胶质细胞培养物暴露于IL-1 β显著增强了这一测量。这种胶质细胞衍生的IL-1 β作为星形胶质细胞生长因子的可能作用通过实验得到证实，实验表明淋巴因子增加了[H-3]胸苷掺入星形胶质细胞，但不是小胶质细胞培养物。与此相反，IL-2没有显着改变胶质细胞增殖。在海马神经元培养中，这些淋巴因子对神经元存活的影响不同。因此，只有最高浓度（500 ng/ml）的IL-1 β测试降低了长期（3天），但不是短期（1天），这些神经元的存活，而神经元的存活是由IL-2即使在短期（1天）暴露。此外，在长期（3天）暴露于IL-2的神经元培养物中，注意到广泛的细胞肿胀、空泡化和神经突回缩，即使在暴露于相对低浓度（
The present study was undertaken to assess the effects of interleukin-1 beta (IL-1 beta) and interleukin-2 (IL-2) on glial and neuronal cells in culture. The presence of IL-1 beta-like and IL-2-like immunoreactivity was detected in media collected from both astroglial and microglial cultures, indicating that both lymphokines can be released from either cell type. However, the levels measured in microglial media were significantly higher than in the astroglial media. Moreover, the content of IL-1 beta-like immunoreactive material in the media was approximately five- to 10-fold greater than that of IL-2, although exposure of both microglial and astroglial cultures to IL-1 beta significantly enhanced this measure. A possible role for this glial-derived IL-1 beta as an astroglial growth factor was substantiated by experiments showing that the lymphokine increased the incorporation of [H-3]thymidine into astroglial, but not microglial cultures. In contrast, 1L-2 did not significantly alter glial proliferation. In hippocampal neuronal cultures, these lymphokines affected neuronal survival differently. Thus, only the highest concentration (500 ng/ml) of IL-1 beta tested decreased the long-term (three day), but not the short-term (one day), survival of these neurons, whereas neuronal survival was compromised by IL-2 even after short-term (one day) exposure. In addition, in the long-term (three-day-old) neuronal cultures exposed to IL-2, extensive cellular swelling, vacuolations and neurite retractions were noted, even in cultures exposed to relatively low concentrations (