Comparative analysis of miRNA profile in human dendritic cells infected with respiratory syncytial virus and human metapneumovirus.
Comparative analysis of miRNA profile in human dendritic cells infected with respiratory syncytial virus and human metapneumovirus.
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DOI：
10.1186/s13104-018-3541-0
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发表时间：
2018-07-03
影响因子：
1.8
通讯作者：
Guerrero-Plata A
中科院分区：
文献类型：
--
作者：
Baños-Lara MDR;Zabaleta J;Garai J;Baddoo M;Guerrero-Plata A
人偏肺病毒（HMPV）和呼吸道合胞病毒（RSV）是呼吸道疾病的主要病原，主要发生在儿童身上。尽管这两种肺炎病毒在临床和流行病学上有相似之处，但它们会引起不同的免疫反应。这项工作旨在通过确定小的非编码RNA（miRNA）的变化来进一步了解这些呼吸道病毒诱导的差异免疫反应，这些小的非编码RNA调节基因表达并参与包括免疫系统在内的许多细胞过程。在本研究中，我们使用Illumina测序技术通过高通量测序分析了RSV或HMPV感染的人树突状细胞的miRNA转录本的表达。通过定量聚合酶链反应进一步验证了miRNA的表达，HMPV感染上调了2种miRNA（hsa-miR-182- 5 p和hsa-miR-4634）的表达，而RSV感染诱导了3种miRNA（hsa-miR-4448、hsa-miR-30 a-5 p和hsa-miR-4634）的显著表达。两种病毒诱导的主要miRNA是hsa-miR-4634。
Human metapneumovirus (HMPV) and respiratory syncytial virus (RSV) are responsible for respiratory diseases, mostly in children. Despite the clinical and epidemiological similarities between these two pneumoviruses, they elicit different immune responses. This work aims to further our understanding of the differential immune response induced by these respiratory viruses by determining the changes of small non-coding RNAs (miRNAs), which regulate gene expression and are involved in numerous cellular processes including the immune system. In the present study, we analyzed the expression of miRNA transcripts of human dendritic cells infected with RSV or HMPV by high throughput sequencing using Illumina sequencing technology. Further validation of miRNA expression by quantitative polymerase chain reaction indicated that HMPV infection up-regulated the expression of 2 miRNAs (hsa-miR-182-5p and hsa-miR-4634), while RSV infection induced significant expression of 3 miRNAs (hsa-miR-4448, hsa-miR-30a-5p and hsa-miR-4634). The predominant miRNA induced by both viruses was hsa-miR-4634.