Effect of the Jianpi Bushen Prescription on the expression of SHP-1, Wnt3a, and AP-1 proteins in chemically damaged mice
Effect of the Jianpi Bushen Prescription on the expression of SHP-1, Wnt3a, and AP-1 proteins in chemically damaged mice
复制标题
健脾补肾方对化学损伤小鼠SHP-1、Wnt3a、AP-1蛋白表达的影响
DOI：
10.4238/2014.april.25.5
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发表时间：
2014-01-01
影响因子：
0.4
通讯作者：
Tu, Y.
中科院分区：
文献类型：
--
作者：
He, D. C.;Xiao, J. J.;Tu, Y.
研究健脾补肾方对化学损伤模型小鼠3种主要蛋白表达的影响。这3种蛋白是AP-1蛋白家族的Wnt 3a、SHP-1和转录因子（NF-E2、c-jun和c-fos）。昆明种小鼠随机分为化学损伤组（N = 48）和对照组（N = 12），化学损伤组给予环磷酰胺（100 mg/kg）腹腔注射，每日1次，连续3 d，对照组给予等量生理盐水。然后将化学损伤小鼠随机分为化学损伤模型组（N = 12）、阳性对照组（N = 12）、复方阿胶浆（EJS）低剂量组（N = 12）、JBP高剂量组（N = 12）：JBP混悬液（200%浓度）0.1g/kg，每日2次，共9天。取各组小鼠双侧股骨和胫骨骨髓。Western blot检测骨髓单个核细胞中特异性蛋白和转录因子的蛋白表达。结果显示，与对照组相比，化学损伤模型组Wnt 3a蛋白表达明显下调（P < 0.05）。与化学损伤模型组相比，JBP低剂量组、JBP高剂量组和阿胶浆组均显著上调Wnt 3a蛋白表达（P < 0.05），其中JBP低剂量组效果最好。与对照组相比，化学损伤模型组SHP-1、c-fos、c-jun和NF-E2蛋白表达均显著升高（均P < 0.05）。与化学损伤模型组相比，化学损伤模型+小剂量JBP组、化学损伤模型+大剂量JBP组、化学损伤模型+ EJS组SHP-1、c-fos、c-jun、NF-E2蛋白表达均显著降低（P均< 0.05），以小剂量JBP组效果最好。这些结果表明JBP调节化学损伤小鼠中SHP-1、Wnt 3a和AP-1蛋白的表达。
This study investigated the effect of the Jianpi Bushen Prescription (JBP) on the expression of 3 major proteins in chemically damaged model mice. The 3 proteins were the Wnt3a, the SHP-1, and the transcription factors (NF-E2, c-jun, and c-fos) of the AP-1 protein family. Kunming mice were randomly divided into chemically damaged group (N = 48), which received an abdominal injection of (100 mg/kg) cyclophosphamide once a day for 3 consecutive days, and control group (N = 12), which received the same amount of saline. Then, the chemically damaged mice were randomly divided into chemically damaged model group (N = 12), which received 0.2 mL/10 g of saline twice a day for 9 days, positive control group (N = 12), which received 0.2 mL/10 g of the e-jiao slurry (EJS) compound twice a day for 9 days, low dose JBP group (N = 12), which received 0.1 g/kg suspension JBP (100% concentration) twice a day for 9 days and high dose JBP group (N = 12), which received 0.1 g/kg suspension JBP (200% concentration) twice a day for 9 days. The bilateral femur and tibia bone marrow were collected from the mice in all groups. The protein expression of the specified proteins and transcription factors in the bone marrow mononuclear cells were detected by Western blot analysis. The results showed that the protein expression of Wnt3a was significantly downregulated in the chemically damaged model group compared to the control group (P < 0.05). The low dose JBP, high dose JBP, and e-jiao slurry treatments significantly upregulated the protein expression of Wnt3a compared to the chemically damaged model group (P < 0.05), with the low dose JBP producing the best results. Compared to the control group, the protein expressions of SHP-1, c-fos, c-jun, and NF-E2 were significantly higher in the chemically damaged model group (all P < 0.05). The protein expressions of SHP-1, c-fos, c-jun, and NF-E2 were significantly lower in the chemically damaged model + the low dose JBP, chemically damaged model + high dose JBP, or chemically damaged model + EJS group compared to chemically damaged model (all P < 0.05), with the low dose JBP producing the best results. These results indicate that JBP regulates the expressions of SHP-1, Wnt3a, and AP-1 proteins in chemically damaged mice.