The BB0646 protein demonstrates lipase and haemolytic activity associated with Borrelia burgdorferi, the aetiological agent of Lyme disease.
The BB0646 protein demonstrates lipase and haemolytic activity associated with Borrelia burgdorferi, the aetiological agent of Lyme disease.
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BB0646 蛋白表现出与伯氏疏螺旋体（莱姆病的病原体）相关的脂肪酶和溶血活性。
DOI：
10.1111/j.1365-2958.2011.07932.x
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发表时间：
2012
影响因子：
3.6
通讯作者：
Skare,JonT
中科院分区：
文献类型：
--
作者：
Shaw,DanaK;Hyde,JennyA;Skare,JonT
莱姆病的病原体伯氏疏螺旋体由蜱属蜱传播，如果不治疗，可导致受影响个体的显著发病率。最近的报道表明，伯氏疏螺旋体细胞包膜中的多不饱和脂肪酸是氧化损伤的潜在靶点，这可能是致命的。 B. burgdorfer如何应对这种攻击尚不清楚。 在此，我们报告的证据表明，bb0646代码的脂肪酶，位于bosRoperon和具有特异性的饱和和多不饱和脂肪酸。具体而言，携带脂肪酶突变拷贝的菌株，无论是以插入失活构建体的形式，还是以活性位点内的定点突变的形式，与同基因亲本和反式补体相比，表现出减弱的脂解和溶血表型。体内分析表明，虽然bb0646突变体仍然具有感染性，但螺旋体载量显著低于同基因亲本和互补突变体菌株。综上所述，这些数据表明BB 0646是一种广泛的底物特异性脂肪酶，有助于体外脂解和溶血活性，并且是最佳伯氏疏螺旋体感染所需的。
The etiological agent of Lyme disease,Borrelia burgdorferi, is transmitted by ticks of theIxodesgenus and, if untreated, can cause significant morbidity in affected individuals. Recent reports have shown that polyunsaturated fatty acids in theB. burgdorfericell envelope are potential targets for oxidative damage, which can be lethal. HowB. burgdorferiresponds to this assault is not known. Herein we report evidence thatbb0646codes for a lipase that is located within thebosRoperon and that has specificity for both saturated and polyunsaturated fatty acids. Specifically, strains harbouring mutated copies of the lipase, either in the form of an insertionally inactivated construct or site‐directed mutations within the active site, demonstrated attenuated lipolytic and haemolytic phenotypes when compared with the isogenic parent andtrans‐complements.In vivoanalysis showed that while thebb0646mutant remains infectious, the spirochaetal load is significantly lower than both the isogenic parent and the complemented mutant strains. Taken together, these data demonstrate that BB0646 is a broad substrate specific lipase that contributes to lipolytic and haemolytic activityin vitroand is required for optimalB. burgdorferiinfection.