Monosodium urate crystal-induced prostaglandin synthesis in the rat subcutaneous air pouch.
Monosodium urate crystal-induced prostaglandin synthesis in the rat subcutaneous air pouch.
复制标题
尿酸钠晶体诱导大鼠皮下气囊中前列腺素的合成。
DOI：
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发表时间：
1985
影响因子：
3.7
通讯作者：
P. Roberts
中科院分区：
文献类型：
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作者：
T. Gordon;I. Kowanko;M. James;P. Roberts
采用大鼠滑膜气囊模型研究了尿酸钠晶体诱导的炎症反应。晶体注射后30分钟，囊内可见巨噬细胞样细胞和成纤维细胞样细胞对晶体的吞噬作用，并伴有多形核白细胞的积累。前列腺素PGE2和6-酮- pgf1 α的水平，在一系列的眼袋冲洗中测量，显示出与白细胞数量明显的时间分离，前列腺素的峰值水平出现在白细胞最大积累之前。吲哚美辛完全消除了前列腺素的合成，而没有减少眼袋冲洗中的白细胞计数。我们认为，在这个模型中，袋壁细胞可能是前列腺素的主要来源，而这些介质在白细胞趋化性中没有显著作用。
Monosodium urate crystal-induced inflammation was studied in an air pouch model of rat synovium. Phagocytosis of crystals by macrophage-like and fibroblast-like cells in the pouch lining was observed 30 minutes after crystal injection and preceded polymorphonuclear leukocyte accumulation. Levels of the prostaglandins PGE2 and 6-keto-PGF1 alpha, measured in serial pouch washouts, showed a clear temporal dissociation with leucocyte numbers, with peak levels of the prostanoids occurring before the maximum accumulation of leucocytes. Indomethacin abrogated prostaglandin synthesis completely without reducing leucocyte counts in pouch washouts. We propose that the pouch lining cells may be the primary source of the prostaglandins and these mediators do not have a significant role in leucocyte chemotaxis in this model.