Persistent increase in the amount of aquaporin-5 in the apical plasma membrane of rat parotid acinar cells induced by a muscarinic agonist SNI-2011
Persistent increase in the amount of aquaporin-5 in the apical plasma membrane of rat parotid acinar cells induced by a muscarinic agonist SNI-2011
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DOI：
10.1016/s0014-5793(00)01763-4
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发表时间：
2000-07-21
期刊：
影响因子：
3.5
通讯作者：
Ishida, H
中科院分区：
文献类型：
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作者：
Ishikawa, Y;Skowronski, MT;Ishida, H
SNI-2011以浓度依赖性方式诱导大鼠腮腺腺泡顶端质膜（APM）中水通道蛋白5（AQP 5）的量持续增加。这种诱导被p-F-HHSiD、U 73122、TMB-8或丹曲林抑制，但不被双吲哚马来酰亚胺或H-7抑制，表明作用于Mg毒蕈碱受体的SNI-2011通过[Ca 2 +]（i）升高而不是通过蛋白激酶C的活化诱导AQP 5的易位。相反，乙酰胆碱诱导AQP 5瞬时易位至APM。SNI-2011在细胞外Ca 2+存在下诱导[Ca 2 +]（i）的持久振荡。因此，SNI-2011诱导AQP 5向APM的持久易位，并伴随持续的[Ca 2 +]（i）振荡。(C)2000年欧洲生物化学学会联合会。由Elsevier Science B. V.出版，版权所有。
SNI-2011 induces the long-lasting increase in the amount of aquaporin-5 (AQP5) in apical plasma membranes (APMs) of rat parotid acini in a concentration-dependent manner. This induction was inhibited by p-F-HHSiD, U73122, TMB-8, or dantrolene but not by bisindolmaleimide or H-7, indicating that SNI-2011 acting at Mg muscarinic receptors induced translocation of AQP5 via [Ca2+](i) elevation but not via the activation of protein kinase C, In contrast, acetylcholine induced a transient translocation of AQP5 to APMs. SNI-2011 induces long-lasting oscillations of [Ca2+](i) in the presence of extracellular Ca2+. Thus, SNI-2011 induces a longlasting translocation of AQP5 to APMs coupled with persistent [Ca2+](i) oscillations. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.