Inverse-power-law behavior of cellular motility reveals stromal-epithelial cell interactions in 3D co-culture by OCT fluctuation spectroscopy.
Inverse-power-law behavior of cellular motility reveals stromal-epithelial cell interactions in 3D co-culture by OCT fluctuation spectroscopy.
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DOI：
10.1364/optica.2.000877
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发表时间：
2015-10-20
期刊：
影响因子：
10.4
通讯作者：
Troester MA
中科院分区：
文献类型：
--
作者：
Oldenburg AL;Yu X;Gilliss T;Alabi O;Taylor RM 2nd;Troester MA
已知乳腺癌的进展受到局部微环境中的基质细胞的影响。在这里，我们研究了基质成纤维细胞对3D共培养中类器官内乳腺上皮细胞的原位运动（运动性）的影响，这是使用光学相干断层扫描（OCT）从散斑波动光谱推断的。与布朗运动相反，乳腺细胞运动表现出逆幂律波动谱。我们引入两个互补的度量量化波动谱：幂律指数和一个新的定义的运动幅度，这两个都是信号和位置无关。我们发现，在三维共培养中，基质细胞的幂律指数和运动幅度分别与基质细胞的密度呈正相关（p <0.001）和负相关（p<0.01）。我们还展示了如何使用这些指标来可视化高光谱数据，以观察类器官内的异质性。这构成了OCT检测和成像细胞功能变化的简单而强大的工具。
The progression of breast cancer is known to be affected by stromal cells within the local microenvironment. Here we study the effect of stromal fibroblasts on the in-place motions (motility) of mammary epithelial cells within organoids in 3D co-culture, inferred from the speckle fluctuation spectrum using optical coherence tomography (OCT). In contrast to Brownian motion, mammary cell motions exhibit an inverse power-law fluctuation spectrum. We introduce two complementary metrics for quantifying fluctuation spectra: the power-law exponent and a novel definition of the motility amplitude, both of which are signal- and position-independent. We find that the power-law exponent and motility amplitude are positively (p<0.001) and negatively (p<0.01) correlated with the density of stromal cells in 3D co-culture, respectively. We also show how the hyperspectral data can be visualized using these metrics to observe heterogeneity within organoids. This constitutes a simple and powerful tool for detecting and imaging cellular functional changes with OCT.