Effects of irradiation with narrowband-ultraviolet B on up-regulation of histamine H1 receptor mRNA and induction of apoptosis in HeLa cells and nasal mucosa of rats
Effects of irradiation with narrowband-ultraviolet B on up-regulation of histamine H1 receptor mRNA and induction of apoptosis in HeLa cells and nasal mucosa of rats
复制标题
DOI：
10.1016/j.jphs.2018.08.011
复制
发表时间：
2018-09-01
影响因子：
3.5
通讯作者：
Takeda, Noriaki
中科院分区：
文献类型：
--
作者：
Fujii, Tatsuya;Kitamura, Yoshiaki;Takeda, Noriaki
窄带紫外线B（NB-UVB）光疗用于治疗特应性皮炎。以前，我们报道了用200 mJ/cm（2）的310 nm NB-UVB照射抑制佛波醇-12-肉豆蔻酸酯-13-乙酸酯（PMA）诱导的组胺H-1受体（HIR）基因表达上调，而不诱导HeLa细胞凋亡。然而，NB-UVB照射对鼻部症状的影响尚不清楚。在这里，我们表明，低剂量照射与310 nm NB-UVB减轻鼻症状甲苯2，4-二异氰酸酯（TDI）致敏的过敏模型大鼠。310 nm NB-UVB照射抑制PMA诱导的HeLa细胞HIR mRNA的上调，在75-200 mJ/cm（2）剂量下呈剂量依赖性，在150 mJ/cm（2）剂量下呈可逆性，但不诱导细胞凋亡。而310 nm NB-UVB照射剂量大于200 mJ/cm ~ 2时，则可诱导细胞凋亡。Western blot分析表明，NB-UVB照射对HIR基因表达的抑制作用是通过抑制ERK磷酸化而实现的。在TDI致敏的大鼠中，用310 nm NB-UVB以估计剂量100 mJ/cm（2）每天一次持续3天的鼻内照射抑制TDI诱导的喷嚏和鼻粘膜中HIR mRNA的上调，而不诱导凋亡。结果提示，小剂量NB-UVB经鼻反复照射可作为AR的光疗方法。(C)2018作者Elsevier B. V.代表日本药理学会制作和主办。
Narrowband-ultraviolet B (NB-UVB) phototherapy is used for the treatment of atopic dermatitis. Previously, we reported that irradiation with 200 mJ/cm(2) of 310 nm NB-UVB suppressed phorbol-12-myristate-13-acetate (PMA)-induced up-regulation of histamine H-1 receptor (HIR) gene expression without induction of apoptosis in HeLa cells. However, the effect of NB-UVB irradiation on nasal symptoms is still unclear. Here, we show that low dose irradiation with 310 nm NB-UVB alleviates nasal symptoms in toluene 2,4-diisocyanate (TDI)-sensitized allergy model rats. Irradiation with 310 nm NB-UVB suppressed PMA-induced HIR mRNA up-regulation in HeLa cells dose-dependently at doses of 75-200 mJ/cm(2) and reversibly at a dose of 150 mJ/cm(2) without induction of apoptosis. While, at doses of more than 200 mJ/cm(2), irradiation with 310 nm NB-UVB induced apoptosis. Western blot analysis showed that the suppressive effect of NB-UVB irradiation on HIR gene expression was through the inhibition of ERK phosphorylation. In TDI-sensitized rat, intranasal irradiation with 310 nm NB-UVB at an estimated dose of 100 mJ/cm(2) once a day for three days suppressed TDI-induced sneezes and upregulation of HIR mRNA in nasal mucosa without induction of apoptosis. These findings suggest that repeated intranasal irradiation with low dose of NB-UVB could be clinically used as phototherapy of AR. (C) 2018 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society.