Use of a Maize Advanced Intercross Line for Mapping of QTL for Northern Leaf Blight Resistance and Multiple Disease Resistance
Use of a Maize Advanced Intercross Line for Mapping of QTL for Northern Leaf Blight Resistance and Multiple Disease Resistance
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DOI：
10.2135/cropsci2009.02.0066
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发表时间：
2010-03-01
期刊：
影响因子：
2.3
通讯作者：
Smith, Margaret E.
中科院分区：
文献类型：
--
作者：
Balint-Kurti, Peter J.;Yang, Junyun;Smith, Margaret E.
北方叶枯病[NLB；由 Exserohilum turcicum (Pass) K.J. 引起伦纳德和 E.G. [Suggs] 是美国和全世界玉米 (Zea mays L.) 的一种重要真菌病害。 IBM群体是源自Mo17和B73品系之间杂交的高级间交重组自交系群体，在三个环境（2006年和2007年纽约州奥罗拉和2007年北卡罗来纳州克莱顿）评估了与NLB抗性相关的两个性状，即加权平均疾病（WMD）和潜伏期（IP）以及花期天数（DTA）。总体分析中检测到 bin 2.00/2.01 和 4.08 中的两个 WMD 数量性状位点（QTL）；其中，在单独分析的所有三个环境中仅检测到 bin 4.08 中的 QTL。同样，在所有三种环境和整体分析中，仅检测到 bin 2.02 中的一个 IP QTL。还检测到每个性状的几个特定于环境的 OTL。检测到多个 DTA QTL，在 bin 8.05 中检测到效果最强。抗病性状与开花天数之间的相关性普遍较低。本研究的结果与之前使用 IBM 种群识别其他两种玉米叶部病害的 OTL 的研究结果进行了比较，即南方叶枯病（病原体 Cochliobolus heterostropus (Drechs.) Drechs。[无性型 = 玉米双极菌 (Nisikado 和 Miyake) Shoemaker；同义词 = 玉米长蠕孢菌 (Nisikado 和 Miyake)]} 和灰斑病 [病原体] Cercospora zeae-maydis (Tehon 和 E.Y. Daniels)]虽然我们没有发现 QTL 赋予对所有三种疾病的抗性，但在 IBM 群体中发现了对这些疾病的抗性之间的显着相关性，这意味着存在影响对所有三种疾病的抗性的位点（可能还有基因）。
Northern leaf blight [NLB; caused by Exserohilum turcicum (Pass) K.J. Leonard and E.G. Suggs] is an important fungal disease of maize (Zea mays L.) in the United States and worldwide. The IBM population, an advanced intercross recombinant inbred line population derived from a cross between the lines Mo17 and B73, was evaluated in three environments (Aurora, NY, in 2006 and 2007 and Clayton, NC in 2007) for two traits related to NLB resistance, weighted mean disease (WMD) and incubation period (IP), and for days to anthesis (DTA). Two WMD quantitative trait loci (QTL) in bins 2.00/2.01 and 4.08 were detected from the overall analysis; of these, only the QTL in bin 4.08 was detected in all three environments analyzed separately. Likewise, only one IP QTL, in bin 2.02, was detected in all three environments and from the overall analysis. Several environment-specific OTL for each trait were also detected. Several DTA QTL were detected with the strongest effect detected in bin 8.05. Correlations between disease resistance traits and days to anthesis were uniformly low. The results from this study were compared to those of previous studies that used the IBM population to identify OTL for two other maize foliar diseases, southern leaf blight (causal agent Cochliobolus heterostrophus (Drechs.) Drechs. [anamorph = Bipolaris maydis (Nisikado and Miyake) Shoemaker; synonym = Helminthosporium maydis (Nisikado and Miyake)]} and gray leaf spot [causal agent Cercospora zeae-maydis (Tehon and E.Y. Daniels)]. Although we did not find QTL conferring resistance to all three diseases, significant correlations between resistances to these diseases in the IBM population were identified, implying the existence of loci (and possibly genes) affecting resistance to all three diseases.