The meiosis-specific hop2 protein of S-cerevisiae ensures synapsis between homologous chromosomes
The meiosis-specific hop2 protein of S-cerevisiae ensures synapsis between homologous chromosomes
复制标题
DOI：
10.1016/s0092-8674(00)81480-4
复制
发表时间：
1998-08-07
期刊：
影响因子：
64.5
通讯作者：
Roeder, GS
中科院分区：
文献类型：
--
作者：
Leu, JY;Chua, PR;Roeder, GS
酿酒酵母的hop2突变体呈现出一种新的表型：减数分裂染色体形成几乎野生型数量的联会复合体，但大多数染色体与非同源伙伴发生联会。减数分裂特异性的Hop2蛋白在联会之前和联会期间定位于染色体上，并且在起始重组的双链断裂缺失的情况下也是如此。hop2菌株维持野生型水平的减数分裂双链断裂，但这些断裂未被修复。hop2突变体在减数分裂前期的粗线期停滞，RecA样蛋白Dmc1位于联会染色体的众多位点上。我们提出Hop2蛋白的功能是防止非同源染色体之间的联会。
The hop2 mutant of S. cerevisiae displays a novel phenotype: meiotic chromosomes form nearly wild-type amounts of synaptonemal complex, but most chromosomes are engaged in synapsis with nonhomologous partners. The meiosis-specific Hop2 protein localizes to chromosomes prior to and during synapsis and in the absence of the double-strand breaks that initiate recombination. hop2 strains sustain a wild-type level of meiotic double-strand breaks, but these breaks remain unrepaired. The hop2 mutant arrests at the pachytene stage of meiotic prophase with the RecA-like protein Dmc1 located at numerous sites along synapsed chromosomes. We propose that the Hop2 protein functions to prevent synapsis between nonhomologous chromosomes.