NUMBER AND ORGANIZATION OF ACTIN-RELATED SEQUENCES IN THE MOUSE GENOME
NUMBER AND ORGANIZATION OF ACTIN-RELATED SEQUENCES IN THE MOUSE GENOME
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DOI：
10.1016/s0022-2836(83)80035-7
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发表时间：
1983-01-01
影响因子：
5.6
通讯作者：
BUCKINGHAM, ME
中科院分区：
文献类型：
--
作者：
MINTY, AJ;ALONSO, S;BUCKINGHAM, ME
使用含有与2种小鼠横纹肌肌动蛋白mRNA（pAF 81，pAM 91）和非肌肉肌动蛋白mRNA（pAL 41）互补的c[互补]DNA序列的重组质粒来检查小鼠基因组中肌动蛋白相关序列的数量和组织。在限制性小鼠DNA的Southern印迹上检测到大量（< 20个）肌动蛋白相关序列，其中大多数与肌动蛋白编码序列的5“和3”末端杂交，即使在仅显示与肌动蛋白cDNA探针> 80%同源的序列的条件下。对这些印迹进行更严格的洗涤表明，2种横纹肌肌动蛋白各自由单基因编码，而非肌肉（β）肌动蛋白由单基因编码。或γ）actin cDNA在小鼠DNA中检测到1个同源序列和2个密切相关的序列。重组近交系小鼠品系中2个横纹肌肌动蛋白基因的分离表明这些基因不紧密连锁（> 1厘摩），并且骨骼肌肌动蛋白基因不与非肌肉肌动蛋白基因连锁。筛选克隆在Charon 4A中的小鼠基因组DNA库，表明小鼠基因组中肌动蛋白相关序列的数量远高于20。分离了5个cDNA，它们代表20-50个相似但不相同的序列的亚家族的一部分，仅与肌动蛋白cDNA探针（可能是肌动蛋白假基因家族）弱同源，这是最近扩增> 17 × 10 - 6的结果。小鼠DNA的103碱基区域。
Recombinant plasmids containing c[complementary]DNA sequences complementary to the 2 mouse striated-muscle actin mRNA (pAF81, pAM91) and to a non-muscle actin mRNA (pAL41) were used to examine the number and organization of actin-related sequences in the mouse genome. A large number (< 20) of actin-related sequences are detected on Southern blots of restricted mouse DNA, the majority of which hybridize to both the 5'' and 3'' ends of the actin-coding sequence, even under conditions revealing only sequences > 80% homologous to the actin cDNA probes. More stringent washing of these blots indicates that the 2 striated muscle actins are each encoded by single genes, and that a non-muscle (.beta. or .gamma.) actin cDNA detects 1 homologous and 2 closely related sequences in mouse DNA. The segregation of the 2 striated-muscle actin genes in recombinant inbred mouse strains shows that these genes are not closely linked (> 1 centimorgan), and that the skeletal muscle actin gene is not linked to a non-muscle actin gene. Screening a bank of mouse genomic DNA, cloned in Charon 4A, indicates that the number of actin-related sequences in the mouse genome is much higher than 20. Five phages were isolated representing part of a sub-family of 20-50 similar but non-identical sequences, only weakly homologous to actin cDNA probes (probably a family of actin pseudogenes), which are the result of a recent amplification of a > 17 .times. 103 base region of mouse DNA.