Further advances in the production of membrane proteins in Pichia pastoris.
Further advances in the production of membrane proteins in Pichia pastoris.
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DOI：
10.4161/bioe.23886
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发表时间：
2013-11
期刊：
影响因子：
4.9
通讯作者：
Hedfalk K
中科院分区：
文献类型：
--
作者：
Hedfalk K
膜蛋白具有重要的细胞功能，因此在学术界和工业界都具有很高的兴趣。已经做出了许多努力来生产这些目标，其产率允许旨在获得高分辨率结构和机理理解的结晶实验。生产的第一步提供了一个关键的障碍，但我们现在看到的是在相对较短的时间内膜蛋白结构确定的巨大进展。在重组蛋白生产方面的成就对于这一发展至关重要，并且酵母毕赤酵母是真核细胞膜蛋白最常用的宿主。高分辨率结构很好地说明了蛋白质生产的成功，这是Ramón和Marin在2011年的综述“巴斯德毕赤酵母膜蛋白生产的进展”中使用的方法。在这里，真核细胞膜蛋白的生产和结晶的其他进展进行了描述和反思。
Membrane proteins have essential cellular functions and are therefore of high interest in both academia and industry. Many efforts have been made on producing those targets in yields allowing crystallization experiments aiming for high resolution structures and mechanistic understanding. The first step of production provides a crucial barrier to overcome, but what we now see, is great progress in membrane protein structural determination in a relatively short time. Achievements on recombinant protein production have been essential for this development and the yeast Pichia pastoris is the most commonly used host for eukaryotic membrane proteins. High-resolution structures nicely illustrate the successes in protein production, and this is the measure used by Ramón and Marin in their review “Advances in the production of membrane proteins in Pichia pastoris” from 2011. Here, additional advances on production and crystallization of eukaryotic membrane proteins are described and reflected on.
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影响因子：
64.8
作者：
Hansen, Scott B.;Tao, Xiao;MacKinnon, Roderick
通讯作者：
MacKinnon, Roderick
DOI：
10.1126/science.1228757
发表时间：
2012-12-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hou X;Pedi L;Diver MM;Long SB
通讯作者：
Long SB
影响因子：
64.8
作者：
Long, Stephen B.;Tao, Xiao;MacKinnon, Roderick
通讯作者：
MacKinnon, Roderick
影响因子：
56.9
作者：
Miller, Alexandria N.;Long, Stephen B.
通讯作者：
Long, Stephen B.
影响因子：
64.8
作者：
Hino, Tomoya;Arakawa, Takatoshi;Iwanari, Hiroko;Yurugi-Kobayashi, Takami;Ikeda-Suno, Chiyo;Nakada-Nakura, Yoshiko;Kusano-Arai, Osamu;Weyand, Simone;Shimamura, Tatsuro;Nomura, Norimichi;Cameron, Alexander D.;Kobayashi, Takuya;Hamakubo, Takao;Iwata, So;Murata, Takeshi
通讯作者：
Murata, Takeshi