FRET-Mediated Long-Range Wavelength Transformation by Photoconvertible Fluorescent Proteins as an Efficient Mechanism to Generate Orange-Red Light in Symbiotic Deep Water Corals.
FRET-Mediated Long-Range Wavelength Transformation by Photoconvertible Fluorescent Proteins as an Efficient Mechanism to Generate Orange-Red Light in Symbiotic Deep Water Corals.
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DOI：
10.3390/ijms18071174
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发表时间：
2017-07-04
影响因子：
5.6
通讯作者：
Wiedenmann J
中科院分区：
文献类型：
--
作者：
Bollati E;Plimmer D;D'Angelo C;Wiedenmann J
光转换荧光蛋白（photoconvertible fluorescent protein，pcRFPs）是一类在近紫外光照射下发生不可逆的绿-红转换的荧光蛋白。尽管它们在生物技术中的广泛应用，高水平的表达pcRFPs中mesophotic和深度generalist珊瑚物种目前缺乏生物学解释。此外，近紫外波长在水中的渗透减少提出了一个问题，即光驱动的光转换是否与介光区相关，或者体内翻译后色素修饰是否涉及不同的机制。在这里，我们在一个长期的围隔实验中表明，体内的光转换完全依赖于近紫外波长。然而，近紫外线强度相当于中光水下光场在80米的深度是足以驱动的过程中，在体外，这表明光转换可以发生在这些珊瑚的分布下限附近。此外，活珊瑚菌落显示出有效的福斯特共振能量转移（FRET）的证据。我们模拟的介光光场使pcRFP池在体内保持部分光转换状态，使四聚体内FRET最大化，并产生比其他天然RFP具有更高量子产率的长程波长转换系统。我们假设，蓝色波长的有效转换，丰富的深度，橙红色的光可能构成珊瑚的生活在光有限的环境中的适应。
Photoconvertible fluorescent proteins (pcRFPs) are a group of fluorophores that undergo an irreversible green-to-red shift in emission colour upon irradiation with near-ultraviolet (near-UV) light. Despite their wide application in biotechnology, the high-level expression of pcRFPs in mesophotic and depth-generalist coral species currently lacks a biological explanation. Additionally, reduced penetration of near-UV wavelengths in water poses the question whether light-driven photoconversion is relevant in the mesophotic zone, or whether a different mechanism is involved in the post-translational pigment modification in vivo. Here, we show in a long-term mesocosm experiment that photoconversion in vivo is entirely dependent on near-UV wavelengths. However, a near-UV intensity equivalent to the mesophotic underwater light field at 80 m depth is sufficient to drive the process in vitro, suggesting that photoconversion can occur near the lower distribution limits of these corals. Furthermore, live coral colonies showed evidence of efficient Förster Resonance Energy Transfer (FRET). Our simulated mesophotic light field maintained the pcRFP pool in a partially photoconverted state in vivo, maximising intra-tetrameric FRET and creating a long-range wavelength conversion system with higher quantum yield than other native RFPs. We hypothesise that efficient conversion of blue wavelengths, abundant at depth, into orange-red light could constitute an adaptation of corals to life in light-limited environments.
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影响因子：
3.7
作者：
Alieva NO;Konzen KA;Field SF;Meleshkevitch EA;Hunt ME;Beltran-Ramirez V;Miller DJ;Wiedenmann J;Salih A;Matz MV
通讯作者：
Matz MV
DOI：
10.1073/pnas.97.22.11984
发表时间：
2000-10-24
影响因子：
11.1
作者：
Baird, GS;Zacharias, DA;Tsien, RY
通讯作者：
Tsien, RY
影响因子：
16
作者：
Mizuno, H;Mal, TK;Miyawakil, A
通讯作者：
Miyawakil, A
影响因子：
3.4
作者：
Gordon, GW;Berry, G;Herman, B
通讯作者：
Herman, B
影响因子：
10.7
作者：
Field, Steven F.;Matz, Mikhail V.
通讯作者：
Matz, Mikhail V.