Covalent labeling of a high-affinity, guanyl nucleotide sensitive parathyroid hormone receptor in canine renal cortex.
Covalent labeling of a high-affinity, guanyl nucleotide sensitive parathyroid hormone receptor in canine renal cortex.
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犬肾皮质中高亲和力、鸟苷酸敏感的甲状旁腺激素受体的共价标记。
DOI：
10.1021/bi00381a013
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发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
Arnaud,CD
中科院分区：
文献类型：
--
作者：
Nissenson,RA;Karpf,D;Bambino,T;Winer,J;Canga,M;Nyiredy,K;Arnaud,CD
材料和方法 材料。苯甲磺酰氟、胃酶抑素、亮肽素和 NEM 购自 Sigma Chemical Co.（密苏里州圣路易斯）。 Gpp (NH) p、GMP、GDP/3S 和 App-(NH) p 来自 Boehringer Mannheim Biochemicals (In-dianapolis, IN)，HSAB 来自 Pierce Chemical Corp.(Rockford, IL)，抑肽酶来自 Mobay Chemical Corp.(New York, NY)。牛 PTH (l-34)(5000 单位/毫克)1 缩写：PTH，甲状旁腺激素； Mx，分子量； HSAB，7V-羟基琥珀酰亚胺基4-叠氮基苯甲酸酯； b、牛； Tris-HCl、三(羟甲基)氨基甲烷盐酸盐； HEPES，A'-(2-羟乙基)哌嗪-A"-2-乙磺酸；BSA，牛血清白蛋白；SDS-PAGE，十二烷基硫酸钠-聚丙烯酰胺凝胶电泳；EDTA，乙二胺四乙酸三钠盐；GTP，鸟苷5'-三磷酸； GMP，5'-单磷酸鸟苷； Gpp-(NH)p,5'-鸟苷基亚胺二磷酸酯； GDP/3S，鸟苷 5'-0-(2-硫代二磷酸)； App(NH)p,5'-腺苷酰亚胺二磷酸酯； NEM，N-乙基马来酰亚胺； PMSF，苯甲磺酰基 氟化物。
Materials and Methods Materials. Phenylmethanesulfonyl fluoride, pepstatin, leupeptin, and NEM were purchased from Sigma Chemical Co.(St. Louis, MO). Gpp (NH) p, GMP, GDP/3S, and App-(NH) p were from Boehringer Mannheim Biochemicals (In-dianapolis, IN), HSAB was from Pierce Chemical Corp.(Rockford, IL), and aprotinin was from Mobay Chemical Corp.(New York, NY). Bovine PTH (l-34)(5000 units/mg)1 Abbreviations: PTH, parathyroid hormone; Mx, molecular weight; HSAB, 7V-hydroxysuccinimidyl 4-azidobenzoate; b, bovine; Tris-HCl, tris (hydroxymethyl) aminomethane hydrochloride; HEPES, A'-(2-hydroxyethyl) piperazine-A"-2-ethanesulfonic acid; BSA, bovine serum albumin; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gelelectrophoresis; EDTA, ethylenediaminetetraacetic acid trisodium salt; GTP, guanosine 5'-triphosphate; GMP, guanosine 5'-monophosphate; Gpp-(NH) p, 5'-guanylyl imidodiphosphate; GDP/3S, guanosine 5'-0-(2-thio-diphosphate); App (NH) p, 5'-adenylyl imidodiphosphate; NEM, N-ethylmaleimide; PMSF, phenylmethanesulfonyl fluoride.