UAUG and uORFs in human and rodent 5′untranslated mRNAs
UAUG and uORFs in human and rodent 5′untranslated mRNAs
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DOI：
10.1016/j.gene.2004.11.041
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发表时间：
2005-04-11
期刊：
影响因子：
3.5
通讯作者：
Pesole, G
中科院分区：
文献类型：
--
作者：
Iacono, M;Mignone, F;Pesole, G
翻译控制是基因表达调控的基本机制。在翻译调控中起作用的顺式作用元件包括位于mRNA的5 UTR中的上游开放阅读框（uORF）和上游AUG（uAUG）。我们在这里提出了一个全基因组分析的uAUG和uORF在一组策划的人类和啮齿动物的mRNA。我们的研究表明，uAUG的出现比uORF的出现受到更强烈的抑制，并且框内uAUG比框外uAUG受到更强烈的抑制。在小鼠mRNA中也观察到非常相似的uAUG/uORF频率模式。orthopedia 5 UTR序列的分析揭示了一个显着程度的进化保守性只有那些uORF获得了一些功能活性。我们的数据表明，除了泄漏扫描和重新启动，这可能会发生与变量和基因特异性的效率，核糖体分流机制，最终耦合到重新启动后uORF翻译，可能是一种普遍的模式，在真核生物中的翻译调控。(c)2004 Elsevier B. V.保留所有权利。
The control of translation is a fundamental mechanism in the regulation of gene expression. Among the cis-acting elements that play a role in translation regulation are upstream open reading frames (uORFs) and upstream AUG (uAUGs) located in the 5UTR of mRNAs. We present here a genome-wide analysis of uAUGs and uORFs in a curated set of human and rodent mRNAs. Our study shows that the occurrence of uAUGs is suppressed more strongly than that of uORFs and that in-frame uAUGs are more strongly suppressed than out-of-frame uAUGs. A very similar pattern of uAUG/uORF frequency was also observed in mouse mRNAs. The analysis of orthologous 5UTR sequences revealed a remarkable degree of evolutionary conservation only of those uORFs which acquired some functional activity. Our data suggest that besides leaky scanning and reinitiation, which likely occur with variable and gene-specific efficiency, the ribosome-shunt mechanism, eventually coupled to reinitiation after uORF translation, may be a widespread mode of translation regulation in eukaryotes. (c) 2004 Elsevier B.V. All rights reserved.