Functional similarity between adenovirus E1B 19-kDa protein and proteins encoded by Bcl-2 proto-oncogene and Epstein-Barr virus BHRF1 gene.
Functional similarity between adenovirus E1B 19-kDa protein and proteins encoded by Bcl-2 proto-oncogene and Epstein-Barr virus BHRF1 gene.
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腺病毒 E1B 19-kDa 蛋白与 Bcl-2 原癌基因和 Epstein-Barr 病毒 BHRF1 基因编码的蛋白之间的功能相似性。
DOI：
10.1007/978-3-642-79496-4_9
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发表时间：
1995
影响因子：
--
通讯作者：
Chinnadurai,G
中科院分区：
文献类型：
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作者：
Subramanian,T;Tarodi,B;Chinnadurai,G
E1B 19 kDa 编码区内一类突变体的分离和物理定位，被称为 2 型腺病毒 (Ad) 大噬斑 (Ip) 突变体（C 组），为 E1B 19-kDa 蛋白的功能提供了初步线索（Chinnaduraiet al. 1979；Chinnadurai 1983）。 Takemori 及其同事 (Takemoriet al. 1968) 分离出一类名为 Ad12（A 组）杀细胞 (cyt) 突变体的突变体，该突变体也在受感染的细胞单层上产生大噬斑，随后通过型间突变互补将其定位到 E1B 19 kDa 编码区 (Subramanianet al. 1984b)。 Ad12 的细胞色素突变体对受感染的细胞产生广泛的细胞病变效应 (Takemoriet al. 1968)。正如预期的那样，随后的研究表明，19-kDa 蛋白缺陷的几个 Ad2 和 Ad5 突变体（19K 突变体）也会诱导严重的细胞病变效应（Subramanianet al. 1984b；Takemoriet al. 1984）。根据 Mak 及其同事（Ezoeet al. 1981）的观察，病毒 DNA 在感染 Ad12 cyt 突变体的细胞中被广泛降解，还发现一些 Ad2/Ad5 19K 突变体在受感染的细胞中诱导细胞和病毒 DNA (deg) 断裂（Subramanianet al. 1984a；Pilderet al. 1984；Whiteet al. 1984a）。遗传学研究表明，cyt 和 deg 表型是相关的，从而表明细胞和病毒 DNA 的断裂是 19K 突变体诱导的细胞病变效应的结果 (Subramanianand Chinnadurai1986)。
The isolation and physical mapping of a class of mutants designated large plaque (Ip) mutants of adenovirus (Ad) type 2 (group C) within the E1B 19 kDa- coding region provided initial clues about the functions of E1B 19-kDa protein (Chinnaduraiet al. 1979; Chinnadurai1983). A class of mutants designated the cytocidal (cyt) mutants of Ad12 (group A) isolated by Takemori and colleagues (Takemoriet al. 1968) which also produce large plaques on infected cell monolayers was subsequently mapped to the E1B 19 kDa-coding region by intertypic mutant complementation (Subramanianet al. 1984b). The cyt mutants of Ad12 induce extensive cytopathic effect on infected cells (Takemoriet al. 1968). As expected, subsequent studies revealed that several Ad2 and Ad5 mutants defective in the 19-kDa protein (19K mutants) also induced severe cytopathic effects (Subramanianet al. 1984b; Takemoriet al. 1984). In accordance with an observation by Mak and colleagues (Ezoeet al. 1981 ) that viral DNA is extensively degraded in cells infected with Ad12 cyt mutants, several Ad2/Ad5 19K mutants were also found to induce fragmentation of cellular and viral DNA (deg) in infected cells (Subramanianet al. 1984a; Pilderet al. 1984; Whiteet al. 1984a). Genetic studies have indicated that the cyt and deg phenotypes are linked, thereby indicating that the fragmentation of cellular and viral DNA is a consequence of the cytopathic effect induced by the 19K mutants (Subramanianand Chinnadurai1986).