Evidence for de novo sphingolipid biosynthesis in Toxoplasma gondii
Evidence for de novo sphingolipid biosynthesis in Toxoplasma gondii
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DOI：
10.1016/s0020-7519(02)00009-7
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发表时间：
2002-06-01
影响因子：
4
通讯作者：
Schwarz, RT
中科院分区：
文献类型：
--
作者：
Azzouz, N;Rauscher, B;Schwarz, RT
糖脂是细胞膜的重要组成部分，参与多种生物学功能。在这份报告中，我们描述了弓形虫速殖子从头合成鞘糖脂的鉴定。用氚丝氨酸和半乳糖对寄生虫进行代谢标记，鉴定寄生虫特异性糖脂。根据标记方案和它们对碱处理的不敏感性，这些糖脂被表征为鞘磷脂。Threo-phenyl-2-palmitoylamino-3-morpholino-1-propanol和L-环丝氨酸这两种公认的神经鞘糖脂生物合成的抑制剂可以抑制寄生虫鞘糖脂的合成。已鉴定的糖脂对内糖神经酰胺酶II的处理不敏感，表明它们可能属于球型糖鞘糖脂。综上所述，我们首次提供了弓形虫能够从头合成神经鞘糖脂的证据。(C)2002年，爱思唯尔科学有限公司代表澳大利亚寄生虫学学会出版。
Glycolipids are important components of cellular membranes involved in various biological functions. In this report, we describe the identification of the de novo synthesis of glycosphinolipids by Toxoplasma gondii tachyzoites. parasite-specific glycolipids were identified by metabolic labelling of parasites with tritiated serine and galactose. These glycolipids were characterised as sphingolipids based on the labelling protocol and their insensitivity towards alkaline treatment. Synthesis of parasite glycosphingolipids were inhibited by threo-phenyl-2-palmitoylamino-3-morpholino-1-propanol and L-cycloserine, two well-established inhibitors of de novo sphingolipid biosynthesis. The identified glycolipids were insensitive towards treatment with endoglycoceramidase II indicating that they might belong to globo-type glycosphingolipids. Taken together, we provide evidence for the first time that T. gondii is capable of synthesising glycosphingolipids de novo. (C) 2002 Published by Elsevier Science Ltd. on behalf of Australian Society for Parasitology Inc.