Embryonic-derived glial-restricted precursor cells (GRP cells) can differentiate into astrocytes and oligodendrocytes in vivo
Embryonic-derived glial-restricted precursor cells (GRP cells) can differentiate into astrocytes and oligodendrocytes in vivo
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DOI：
10.1006/exnr.2001.7729
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发表时间：
2001-09-01
影响因子：
5.3
通讯作者：
Mayer-Proschel, M
中科院分区：
文献类型：
--
作者：
Herrera, J;Yang, H;Mayer-Proschel, M
我们从胚胎脊髓中分离并鉴定了一种独特的胶质限制性前体细胞（GRP）。克隆分析表明，当在体外暴露于适当的信号时，这些细胞能够产生少突胶质细胞和两种不同类型的星形胶质细胞（I 型和 2 型）。我们现在证明这些细胞的许多方面都保留在体内。 GRP 细胞在体内仅限于神经胶质谱系，因为它们似乎无法在体内神经原性环境中产生神经元表型。 GRP 细胞在新生儿和成人大脑中存活并迁移。移植的 GRP 细胞在髓磷脂缺乏的背景下分化为形成髓磷脂的少突胶质细胞，并在正常新生儿大脑中产生未成熟的少突胶质细胞。此外，GRP 细胞还在新生儿和成人大脑中持续产生神经胶质纤维蛋白表达细胞，这是其他神经胶质前体细胞（如 O-2A/OPC 细胞）无法持续表达的特性。我们认为，GRP 细胞的谱系限制及其在体内产生少突胶质细胞和星形胶质细胞的能力以及允许群体在体外广泛扩增的胚胎特征使得该细胞可用于临床应用。 (C) 2001 年学术出版社。
We have isolated and characterized a unique glial-restricted precursor cell (GRP) from the embryonic spinal cord. Clonal analysis demonstrated that these cells are able to generate oligodendrocytes and two distinct type of astrocytes (type I and type 2) when exposed to appropriate signals in vitro. We now show that many aspects of these cells are retained in vivo. GRP cells are restricted to the glial lineage in vivo as they seem to be unable to generate neuronal phenotypes in an in vivo neurogenic environment. GRP cells survive and migrate in the neonatal and adult brain. Transplanted GRP cells differentiate into myelin-forming oligodendrocytes in a myelin-deficient background and also generate immature oligodendrocytes in the normal neonatal brain. In addition, GRP cells also consistently generated glial fibrillary protein-expressing cells in the neonatal and adult brain, a property not consistently expressed by other glial precursor cells like the O-2A/OPC cells. We suggest that the lineage restriction of GRP cells and their ability to generate both oligodendrocytes and astrocytes in vivo together with their embryonic character that allows for extensive in vitro expansion of the population makes the cell useful for clinical application. (C) 2001 Academic Press.