Diversity and Distribution of Anaerobic Ammonium Oxidation Bacteria in Hot Springs of Conghua, China.
Diversity and Distribution of Anaerobic Ammonium Oxidation Bacteria in Hot Springs of Conghua, China.
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DOI：
10.3389/fmicb.2021.739234
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发表时间：
2021
影响因子：
5.2
通讯作者：
Li WJ
中科院分区：
文献类型：
--
作者：
Liu L;Lv AP;Narsing Rao MP;Ming YZ;Salam N;Li MM;Liu ZT;Zhang XT;Zhang JY;Xian WD;Jiao JY;Li WJ
厌氧氨氧化（anammox）是氮循环的一个重要过程，厌氧氨氧化细菌在各种环境中的研究都很广泛。然而，温泉中厌氧氨氧化细菌的分布、多样性和丰度仍然是个谜。本研究首次对从化温泉的厌氧氨氧化工艺进行了研究。检测到与“Ammodatus Brocadia”、“Ammodatus Kuenenia”、“Ammodatus Scalindua”、“Ammodatus Anammoxoglobus”和“Ammodatus Jettenia”密切相关的类氨细菌序列。本研究中的几个操作分类单位（OTU）与已知的厌氧氨氧化细菌的16 S rRNA基因具有低序列同一性，表明它们可能代表推定的新型厌氧氨氧化细菌。对厌氧氨氧化菌特异性16 S rRNA基因的定量PCR分析证实，厌氧氨氧化菌的丰度范围为1.60 × 104至1.20 × 107拷贝L-1。硝酸盐是决定温泉生态系统中厌氧氨氧化细菌群落地理分布的关键环境因子。溶解无机碳对厌氧氨氧化细菌多样性有显著影响。本研究首次揭示了从化温泉中存在着多种厌氧氨氧化细菌，包括可能的新的候选厌氧氨氧化细菌，将我国温泉中的厌氧氨氧化细菌的存在范围扩展到了温泉，并扩展了我们对厌氧氨氧化细菌地理学的认识。本研究填补了我国温泉生境中厌氧氨氧化细菌的研究空白，对从化温泉厌氧氨氧化细菌的富集策略具有指导意义。
Anaerobic ammonium oxidation (anammox) is an important process of the nitrogen cycle, and the anammox bacteria have been studied in a wide variety of environments. However, the distribution, diversity, and abundance of anammox bacteria in hot springs remain enigmatic. In this study, the anammox process was firstly investigated in hot springs of Conghua, China. Anammox-like bacterial sequences that closely affiliated to “Candidatus Brocadia,” “Candidatus Kuenenia,” “Candidatus Scalindua,” “Candidatus Anammoxoglobus,” and “Candidatus Jettenia” were detected. Several operational taxonomic units (OTUs) from this study shared low sequence identities to the 16S rRNA gene of the known anammox bacteria, suggesting that they might be representing putative novel anammox bacteria. A quantitative PCR analysis of anammox-specific 16S rRNA gene confirmed that the abundance of anammox bacteria ranged from 1.60 × 104 to 1.20 × 107 copies L–1. Nitrate was a key environmental factor defining the geographical distribution of the anammox bacterial community in the hot spring ecosystem. Dissolved inorganic carbon had a significant influence on anammox bacterial biodiversity. Our findings for the first time revealed that the diverse anammox bacteria, including putative novel anammox bacterial candidates, were present in Conghua hot spring, which extended the existence of anammox bacteria to the hot springs in China and expands our knowledge of the biogeography of anammox bacteria. This work filled up the research lacuna of anammox bacteria in Chinese hot spring habitat and would guide for enrichment strategies of anammox bacteria of Conghua hot springs.