Role of PLLA Plasma Surface Modification in the Interaction with Human Marrow Stromal Cells
Role of PLLA Plasma Surface Modification in the Interaction with Human Marrow Stromal Cells
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DOI：
10.1002/app.31008
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发表时间：
2009-12-15
影响因子：
3
通讯作者：
Kenny, Jose M.
中科院分区：
文献类型：
--
作者：
Armentano, Ilaria;Ciapetti, Gabriela;Kenny, Jose M.
研究了氧基射频等离子体增强化学气相沉积（rf PECVD）在聚l -丙交酯（PLLA）聚合物表面的作用及其对蛋白质吸附和骨细胞行为的影响。制备了基于聚乳酸聚合物的薄膜和多孔支架，并对表面修饰的作用进行了广泛的研究。PECVD表面处理用于改变表面功能和调节蛋白质在聚乳酸聚合物基体上的吸附。特别地，用荧光显微镜分析了牛血清白蛋白荧光素异硫氰酸酯（fitc-BSA）在处理过的PLLA的图案表面上的共轭吸附。在支架上培养人骨髓基质细胞（MSCs），荧光显微镜观察细胞黏附和形态。结果表明，随着处理时间的延长，pla表面亲水性增强，粗糙度增大，对蛋白质的吸附过程有主导影响。等离子体处理各种PLLA表面的结果已被证明是细胞粘附蛋白表达的上调，从而改善细胞的粘附和生长。与未处理的样品相比，氧处理的PLLA促进了MSCs的更高粘附和增殖。可以得出结论，在血浆处理后，PLLA样品对骨祖细胞的亲和力增强。(C) 2009 Wiley期刊公司应用化学学报，2009,31 (2):387 - 398
The effects of oxygen-based radio frequency plasma enhanced chemical vapor deposition (rf PECVD) on the surface of poly(L-lactide) (PLLA) polymers and the influence thereof on protein adsorption and on bone-cell behavior have been studied. Thin films and porous scaffolds based on PLLA polymer were developed, and the role of surface modifications were investigated extensively. PECVD surface treatments were used to alter surface functionality and modulate protein adsorption on the PLLA polymer matrix. In particular, Bovine Serum Albumine fluorescein isothiocyanate (fitc-BSA) conjugate adsorption on patterned surfaces of treated PLLA was analyzed by fluorescence microscopy. Human marrow stromal cells (MSCs) were cultured on scaffolds and cell adhesion and morphology were assessed using fluorescence microscopy. The results indicated that the PLLA surface became hydrophilic and its roughness increased with the treatment time and it had a dominant influence on the adsorption process of the protein. The outcome of the plasma treatment of various PLLA surfaces has been shown to be the up-regulator of the cell-adhesive proteins expression and consequently the improvement of cell adhesion and growth. Oxygen-treated PLLA promoted higher adhesion and proliferation of the MSCs in comparison to the untreated samples. It can be concluded that following plasma treatment, PLLA samples show enhanced affinity for osteoprogenitor cells. (C) 2009 Wiley Periodicals, Inc. J Appl Polym Sci 114: 3602-3611, 2009