A high ratio of dietary n-3/n-6 polyunsaturated fatty acids improves obesity-linked inflammation and insulin resistance through suppressing activation of TLR4 in SD rats
A high ratio of dietary n-3/n-6 polyunsaturated fatty acids improves obesity-linked inflammation and insulin resistance through suppressing activation of TLR4 in SD rats
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高比例的膳食 n-3/n-6 多不饱和脂肪酸通过抑制 SD 大鼠中 TLR4 的激活来改善肥胖相关的炎症和胰岛素抵抗
DOI：
10.1016/j.nutres.2013.07.004
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发表时间：
2013-10-01
影响因子：
4.5
通讯作者：
Wang, Feng
中科院分区：
文献类型：
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作者：
Liu, Han-Qiang;Qiu, Ya;Wang, Feng
膳食中n-3/n-6多不饱和脂肪酸(PUFAs)的比例被认为是控制代谢紊乱的标记物，包括肥胖、胰岛素抵抗(IR)、炎症和血脂谱，这些也被认为与2型糖尿病(T2 DM)部分相关。然而，与代谢紊乱相关的不同多不饱和脂肪酸的分子机制还没有得到系统的研究。本研究旨在研究膳食n-3/n-6多不饱和脂肪酸比例对肥胖和胰岛素抵抗的影响，并进一步确定其潜在机制。连续16周，32只雄性SD大鼠随机分为4组，每组8只，分别饲喂普通饲料、高饱和脂肪酸(SFA)、高n-3/n-6蛹比(101，PUFA(1：1))或低n-3/n-6 PUFA(104，PUFA)。在实验饮食期间，测量与肥胖和胰岛素抵抗相关的代谢参数。与SFA饲料喂养的大鼠相比，PUFA(1：1)饲料喂养的大鼠体重和内脏脂肪重量减少，血脂降低，糖耐量和胰岛素敏感性提高。有趣的是，这些变化伴随着循环促炎细胞因子的表达水平降低，包括肿瘤坏死因子α、白细胞介素6和C反应蛋白。此外，与SFA相比，PUFA(1：1)显著下调TLR4的蛋白和mRNA水平，而PUFA(1：4)喂养的大鼠则没有表现出这些变化。总体而言，我们的数据强调了多不饱和脂肪酸(1：1)饮食通过抑制促炎症细胞因子的关键调节因子TLR4的激活，在预防肥胖和相关代谢紊乱方面的作用。(C)2013 Elsevier Inc.保留所有权利。
Dietary ratios of n-3/n-6 polyunsaturated fatty acids (PUFAs) have been implicated in controlling markers of metabolic disorders, including obesity, insulin resistance (IR), inflammation, and lipid profiles, which are also presumed to be partly related to type 2 diabetes mellitus (T2DM). However, molecular mechanisms of the different PUFAs related to metabolic disorders have not been systematically addressed. The present study aimed to investigate the impact of dietary n-3/n-6 PUFA ratios, on obesity and IR and, further, to determine the underlying mechanisms. For 16 weeks, 32 SD male rats, randomly divided into four groups (n = 8 per group), received one of the following diets: normal chow, high saturated fatty acid (SFA), high n-3/n-6 PUPA ratio (101, PUFA(1:1)), or low n-3/n-6 PUFA ratio (104, PUFA(1:4)). Following the experimental diet period, metabolic parameters related to obesity and IR were measured. Compared to SFA diet-fed rats, PUFA(1:1) diet-fed rats exhibited decreased body and visceral fat weight, lowered blood lipids, and improved glucose tolerance and insulin sensitivity. Interestingly, these changes were accompanied with decreased expression levels of circulating pro-inflammatory cytokines, including tumor necrosis factor alpha, interleukin-6, and C-reactive protein. Moreover, the TLR4 protein and mRNA levels were markedly down-regulated by PUFA(1:1) compared with SFA; however, PUFA(1:4) diet-fed rats failed to exhibit these changes. Cumulatively, our data highlight a role for a PUFA(1:1) diet in the prevention of obesity and related metabolic disorders by suppressing the activation of TLR4, a critical modulator of pro-inflammatory cytokines. (C) 2013 Elsevier Inc. All rights reserved.