MOLECULAR DEVELOPMENT OF THE LATERAL GENICULATE NUCLEUS IN THE ABSENCE OF RETINAL WAVES DURING THE TIME OF RETINAL AXON EYE-SPECIFIC SEGREGATION
MOLECULAR DEVELOPMENT OF THE LATERAL GENICULATE NUCLEUS IN THE ABSENCE OF RETINAL WAVES DURING THE TIME OF RETINAL AXON EYE-SPECIFIC SEGREGATION
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DOI：
10.1016/j.neuroscience.2009.02.010
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发表时间：
2009-04-10
期刊：
影响因子：
3.3
通讯作者：
Kawasaki, H.
中科院分区：
文献类型：
--
作者：
Iwai, L.;Kawasaki, H.
当双眼抑制视网膜波时，眼睛特异性的视网膜轴突分离被破坏，并且来自两只眼睛的视网膜轴突在外侧膝状体核（LGN）中保持混合。这种双眼视网膜波抑制的作用是由两只眼睛的视网膜轴突之间缺乏活动依赖性竞争介导的，但尚不清楚这种作用是否也是由处于未成熟状态的 LGN 发育停滞介导的。在这里，我们发现了眼睛特异性分离过程中 LGN 的发育标记。 LGN 中浦肯野细胞蛋白 4 (PCP41 PEP19)、转录因子 7-like 2 (TCF7L2ITCF4) 和 LIM 同源框蛋白 9 (Lhx9) 的表达水平在眼特异性分离过程中发生显着变化。使用 PCP4、TCF7L2 和 Lhx9 作为 LGN 的发育标记，我们检查了 LGN 发育是否受到眼睛特异性分离过程中视网膜波双眼破坏的影响。双眼注射皮巴替丁强烈抑制眼特异性分离，但不影响 PCP4、TCF7L2 和 Lhx9 的表达。此外，在双眼摘除动物和用单胺氧化酶 A (MAOA) 抑制剂氯吉林治疗的小鼠中，PCP4、TCF7L2 和 Lhx9 的表达是正常的。此外，我们使用 LGN 切片培养物的实验表明，LGN 切片中 PCP4 和 TCF7L2 的表达与体内一样发生变化。我们的结果表明，即使在没有视网膜输入的情况下，LGN 的发育至少在一定程度上仍在进行。 PCP4、TCF7L2 和 Lhx9 应有助于检查小鼠和雪貂眼睛特异性分离过程中 LGN 的发育。 (C) 2009 国际广播组织。由爱思唯尔有限公司出版。保留所有权利。
When retinal waves are inhibited binocularly, eye-specific segregation of retinal axons is disrupted, and retinal axons from the two eyes remain intermingled in the lateral geniculate nucleus (LGN). This effect of binocular retinal wave inhibition is mediated by the lack of activity-dependent competition between retinal axons from the two eyes, but it is unknown whether this effect is also mediated by the developmental arrest of the LGN in an immature state. Here we find developmental markers of the LGN during eye-specific segregation. The expression levels of Purkinje cell protein 4 (PCP41 PEP19), transcription factor 7-like 2 (TCF7L2ITCF4) and LIM homeobox protein 9 (Lhx9) in the LGN change significantly during eye-specific segregation. Using PCP4, TCF7L2 and Lhx9 as developmental markers of the LGN, we examine whether LGN development is affected by binocular disruption of retinal waves during eye-specific segregation. Binocular injection of epibatidine strongly inhibits eye-specific segregation, whereas it does not affect the expression of PCP4, TCF7L2 and Lhx9. Furthermore, the expression of PCP4, TCF7L2 and Lhx9 is normal in binocularly enucleated animals and in mice treated with the monoamine oxidase A (MAOA) inhibitor, clorgyline. In addition, our experiments using LGN slice cultures show that the expression of PCP4 and TCF7L2 in LGN slices changes as in vivo. Our results suggest that LGN development proceeds, at least in part, even in the absence of retinal inputs. PCP4, TCF7L2 and Lhx9 should be useful to examine LGN development during eye-specific segregation in mice and in ferrets. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.