Regulation of endometrial endothelial cell proliferation by oestrogen and progesterone in the ovariectomized mouse
Regulation of endometrial endothelial cell proliferation by oestrogen and progesterone in the ovariectomized mouse
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DOI：
10.1530/rep.0.1230107
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发表时间：
2002-01-01
期刊：
影响因子：
3.8
通讯作者：
Rogers, PAW
中科院分区：
文献类型：
--
作者：
Heryanto, B;Rogers, PAW
虽然子宫内膜上皮细胞和间质细胞对雌激素和孕激素的反应已被很好地描述，但对内皮细胞的反应知之甚少。本研究的目的是调查子宫内膜内皮细胞增殖的时间过程中的一个特定的雌激素和孕激素的方案，并比较它与小鼠子宫内膜的基质和上皮细胞的反应。切除成年雌性小鼠卵巢以诱导子宫内膜退化。7天后，根据以下方案给予激素治疗：第1-3天：100 ng雌二醇;第4-6天：10 ng雌二醇和500 μ g孕酮;第7天：100 ng雌二醇和500 μ g孕酮。在激素治疗的每一天，用溴脱氧尿苷注射小鼠（n=5），并在4小时后用缓冲福尔马林灌注固定。用抗溴脱氧尿苷单克隆抗体检测子宫内膜细胞，用抗CD 31抗体检测内皮细胞。卵巢切除后第7天，子宫内膜中发现少量增殖细胞。雌激素处理1天后，在内皮细胞中检测到显著的增殖（0.0%对16.1+/-1.2%，P
Although the endometrial epithelial and stromal cell response to oestrogen and progesterone is well characterized, relatively little is known about the endothelial cell response. The aim of this study was to investigate the time course of endometrial endothelial cell proliferation in response to a specific regimen of oestrogen and progesterone, and to compare it with the stromal and epithelial cell response in mouse endometrium. Adult female mice were ovariectomized to induce endometrial regression. After 7 days, hormonal treatments were given according to the following regimen: days 1-3: 100 ng oestradiol; days 4-6: 10 ng oestradiol and 500 mug progesterone; and day 7: 100 ng oestradiol and 500 mug progesterone. On each day of hormonal treatment, mice (n=5) were injected with bromodeoxyuridine and perfusion fixed 4 h later with buffered formalin. Proliferating endometrial cells were detected by monoclonal antibody against bromodeoxyuridine, and endothelial cells were detected by antibody to CD31. At day 7 after ovariectomy few proliferating cells were found in the endometrium. After 1 day of oestrogen treatment, significant proliferation was detected in the endothelial cells (0.0% versus 16.1+/-1.2%, P