emm and C-repeat region molecular typing of beta-hemolytic Streptococci in a tropical country: implications for vaccine development.
emm and C-repeat region molecular typing of beta-hemolytic Streptococci in a tropical country: implications for vaccine development.
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热带国家 β 溶血性链球菌的 emm 和 C 重复区域分子分型：对疫苗开发的影响。
DOI：
10.1128/jcm.00312-09
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发表时间：
2009
影响因子：
9.4
通讯作者：
Carapetis,JonathanR
中科院分区：
文献类型：
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作者：
Steer,AndrewC;Magor,Graham;Jenney,AdamWJ;Kado,Joseph;Good,MichaelF;McMillan,David;Batzloff,Michael;Carapetis,JonathanR
我们设计了一项研究，以调查分子流行病学的A组链球菌（GAS）和C组和G链球菌（GCS和GGS）疾病在斐济，一个国家，这是已知的链球菌疾病的高负担。对817株分离株（535株GAS和282株GCS/GGS）进行M蛋白N-末端部分的分子分型（emmtyping）。我们还进行了基因分型的C-重复区在769这些分离株，以确定J14序列类型。斐济的emmtypes分布与美国和欧洲的非常不同。临床疾病状态间无优势类型，存在大量的重叠类型。在工业化国家常见的GASemm型，包括emm 1、emm 12和emm 28，在斐济的GAS分离株中没有发现。超过93%的GAS分离株和超过99%的GCS/GGS分离株进行J14序列分型，含有J14.0或J14.1。我们的数据对发展中国家的GAS疫苗开发具有影响，并表明基于M蛋白保守区域的疫苗可能是斐济和其他热带发展中国家的可行选择。
We designed a study to investigate the molecular epidemiology of group A streptococcal (GAS) and group C and G streptococcal (GCS and GGS) disease in Fiji, a country which is known to have a high burden of streptococcal disease. Molecular typing of the N-terminal portion (emmtyping) of the M protein was performed with 817 isolates (535 GAS and 282 GCS/GGS). We also performed genotyping of the C-repeat region in 769 of these isolates to identify J14 sequence types. The profile ofemmtypes for Fiji was very different from that found for the United States and Europe. There were no dominantemmtypes and a large number of overlapping types among clinical disease states. Commonly found GASemmtypes in industrialized countries, includingemm1,emm12, andemm28, were not found among GAS isolates from Fiji. Over 93% of GAS isolates and over 99% of GCS/GGS isolates that underwent J14 sequence typing contained either J14.0 or J14.1. Our data have implications for GAS vaccine development in developing countries and suggest that a vaccine based upon the conserved region of the M protein may be a feasible option for Fiji and potentially for other tropical developing countries.