Effect of prazosin on microvascular perfusion during middle cerebral artery ligation in the rat.
Effect of prazosin on microvascular perfusion during middle cerebral artery ligation in the rat.
复制标题
哌唑嗪对大鼠大脑中动脉结扎期间微血管灌注的影响。
DOI：
10.1161/01.res.63.1.27
复制
发表时间：
1988
影响因子：
20.1
通讯作者：
H. Weiss
中科院分区：
文献类型：
--
作者：
M. Anwar;E. Buchweitz;H. Weiss
研究戊巴比妥麻醉大鼠大脑中动脉结扎1小时后，α - 1-肾上腺素能受体拮抗剂吡唑嗪对总和灌注脑微血管床形态测量指标的影响。我们假设这种药物可以防止儿茶酚胺引起的缺血脑血管收缩。用14c -碘安替比林测定脑血流（CBF），用异硫氰酸-葡聚糖荧光素观察灌注的微血管床。MCA闭塞不改变全身血流动力学或血气参数。mca结扎皮层的CBF平均为29 +/- 15（平均+/- SD） ml/min/100 g，其他脑区为49 +/- 18。哌唑嗪没有显著改变这些CBF值，平均分别为26 +/- 14和48 +/- 10。两组的总毛细血管/mm2没有显著的区域差异。除缺血皮质外，两组在所有检查区域的毛细血管灌注百分比（51 +/- 6%）相似。在mca结扎的皮质中，灌注了22 +/- 8%的毛细血管体积，而哌唑嗪治疗组为49 +/- 8%。哌唑嗪治疗的大鼠微血管灌注百分比增加，尽管CBF也同样减少。哌唑嗪似乎减少了缺血皮质的扩散距离。这可能是由于其α 1-肾上腺素受体阻断活性。
The purpose of this study was to evaluate the effects of prazosin, an alpha 1-adrenoceptor antagonist, on morphometric indexes of the total and perfused cerebral microvascular bed 1 hour after middle cerebral artery (MCA) ligation in pentobarbital-anesthetized rats. We hypothesized that this agent would prevent catecholamine-induced vasoconstriction in the ischemic brain. Cerebral blood flow (CBF) was determined with 14C-iodoantipyrine, and the perfused microvascular bed was visualized using fluorescein isothiocyanate-dextran. MCA occlusion did not alter systemic hemodynamic or blood gas parameters. CBF averaged 29 +/- 15 (mean +/- SD) ml/min/100 g in the MCA-ligated cortex and 49 +/- 18 in the other examined brain regions. Prazosin did not significantly alter these CBF values, averaging 26 +/- 14 and 48 +/- 10, respectively. There were no significant regional differences in total capillaries/mm2 in either group. The percent of the capillaries/mm2 perfused (51 +/- 6%) was similar in the two groups in all examined regions except the ischemic cortex. In the MCA-ligated cortex, 22 +/- 8% of the capillary volume was perfused in comparison with 49 +/- 8% in the prazosin-treated group. Prazosin-treated rats had an increased percentage of their microvasculature perfused despite a similarly reduced CBF. Prazosin appeared to reduce diffusion distances in the ischemic cortex. This might be due to its alpha 1-adrenoceptor blocking activity.