MALONDIALDEHYDE AND GLUTATHIONE PRODUCTION IN ISOLATED-PERFUSED HUMAN AND RAT HEARTS
MALONDIALDEHYDE AND GLUTATHIONE PRODUCTION IN ISOLATED-PERFUSED HUMAN AND RAT HEARTS
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DOI：
10.1161/01.res.73.4.681
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发表时间：
1993-10-01
影响因子：
20.1
通讯作者：
DEJONG, JW
中科院分区：
文献类型：
--
作者：
JANSSEN, M;KOSTER, JF;DEJONG, JW
大量研究表明氧自由基与心肌缺血/再灌注损伤的关系。然而，对人类心脏中的氧化应激知之甚少，其可以通过氧化型谷胱甘肽（GSSG）和丙二醛（MDA）的形成来测量。此外，大鼠心脏MDA生成的数据是有争议的，可能是因为使用了非特异性硫代巴比妥酸测定。因此，GSSG和MDA的测定，比色法和高效液相色谱法，分别在缓冲液灌注的人心脏和正常大鼠心脏暂时缺血。人的心脏接受心脏停搏液，大鼠心脏进行了研究，在对照组和缺血组或无心脏停搏液。基线GSSG释放为
A number of studies show the relation between oxygen-derived free radicals and cardiac ischemia/reperfusion injury. However, little is known about oxidative stress in the human heart, which can be measured by oxidized glutathione (GSSG) and malondialdehyde (MDA) formation. Furthermore, data on MDA production by rat hearts are controversial, possibly because of the use of the aspecific thiobarbituric acid assay. Therefore, GSSG and MDA were measured, with colorimetric and high-performance liquid chromatographic assays, respectively, in buffer-perfused explanted human hearts and normal rat hearts made temporarily ischemic. Human hearts received cardioplegia; rat hearts were studied in a control and an ischemic group with or without cardioplegia. Baseline GSSG release was