LAUTROPIA MIRABILIS GEN-NOV, SP-NOV, A GRAM-NEGATIVE MOTILE COCCUS WITH UNUSUAL MORPHOLOGY ISOLATED FROM THE HUMAN MOUTH
LAUTROPIA MIRABILIS GEN-NOV, SP-NOV, A GRAM-NEGATIVE MOTILE COCCUS WITH UNUSUAL MORPHOLOGY ISOLATED FROM THE HUMAN MOUTH
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DOI：
10.1099/13500872-140-7-1787
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发表时间：
1994-07-01
期刊：
影响因子：
2.8
通讯作者：
YING, YT
中科院分区：
文献类型：
--
作者：
GERNERSMIDT, P;KEISERNIELSEN, H;YING, YT
在人类上呼吸道标本中重新发现了一种似乎与Orskov的‘Sarcina mirabilis’[Orskov，J.(1930)Acta Pathol Microbiol Scand Suppl III，519-541]相同的微生物。对6个菌株进行了研究，结果与Orskov对奇异链球菌的描述一致。粗糙到光滑的菌落生长在许多平板培养基上，细胞形态呈高度多态，细胞呈圆形，直径从1到10微米。最小的细胞通常运动，呈圆形运动。革兰氏阴性，兼性厌氧，酶、尿素酶阳性，过氧化氢酶弱阳性。还原硝酸盐和亚硝酸盐，发酵葡萄糖、果糖、蔗糖和甘露醇。以蔗糖琼脂为底物生产多糖。电子显微镜显示球状细胞有3至9个鞭毛，革兰氏阴性细胞壁形态，不规则细胞聚集体由共同的表面层聚集在一起。微生物的平均摩尔百分比(G+C)为65.0。S-16-核糖体核糖核酸测序表明，该菌属于变形杆菌的Beta亚群，与所有已描述的属分开，但与伯克霍尔德氏菌关系最近。为这种生物建议命名为奇异月桂。
An organism that seems to be identical to Orskov's 'Sarcina mirabilis' [Orskov, J. (1930) Acta Pathol Microbiol Scand Suppl III, 519-541] has been rediscovered in specimens from the upper respiratory tract of humans. Six strains were studied, and the results, which conformed to Orskov's description of S. mirabilis, were as follows. Rough to smooth colonies grow on many plated media and show extremely polymorphic cell morphology with round cells with diameters from 1 to > 10 mu m. The smallest cells were often motile with circular movements. Strains were Gram-negative, facultatively anaerobic, oxidase and urease positive, and weakly catalase positive. Nitrate and nitrite were reduced, and glucose, fructose, sucrose and mannitol were fermented. Polysaccharide was produced on sucrose agar. Electron microscopy showed coccoid cells with a bundle of three to nine flagella, a Gram-negative cell-wall morphology, and aggregates of irregular cells held together by a common surface layer. The mean mol% (G + C) of the organisms was 65.0. S-16-ribosomal RNA sequencing revealed that the organism belongs to the beta subgroup of Proteobacteria, separate from all other described genera, but most closely related to Burkholderia. The name Lautropia mirabilis is proposed for this organism.