Ecofriendly production of bioactive tissue engineering scaffolds derived from egg- and sea-shells
Ecofriendly production of bioactive tissue engineering scaffolds derived from egg- and sea-shells
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DOI：
10.1016/j.jmrt.2020.09.093
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发表时间：
2020-11-01
影响因子：
6.4
通讯作者：
Rangari, Vijaya K.
中科院分区：
文献类型：
--
作者：
Hembrick-Holloman, Vincent;Samuel, Temesgen;Rangari, Vijaya K.
管理每年全球产生的大量蛋壳和贝壳废物变得非常困难，因为这些材料只是在垃圾填埋场处理。这种废物导致气味产生和微生物生长，直接导致环境压力。将这种废物转化为有价值的生物材料具有显著的环境和经济优势，因为丢弃的废物具有可以利用的材料。这项工作的目的是开发廉价的，生物活性的羟基磷灰石为基础的支架组织工程应用，通过使用环保和可持续的方法。采用高效微波辅助湿化学沉淀法从废弃的鸡蛋壳和文蛤壳中制备工程化纳米羟基磷灰石。采用X射线衍射（XRD）、场发射扫描电子显微镜（FESEM）、透射电子显微镜（TEM）和能量色散谱（EDS）对生物材料进行分析。结果表明，所制备的颗粒为高度结晶的羟基磷灰石（HA），具有针状形状，尺寸在纳米范围内。化学成分与天然HA完全匹配。使用基于浆料的溶液3D打印技术制造EnHA注入的聚合物支架。浆料溶液由70重量%的EnHA和30重量%的聚己内酯（PCL）组成。进行XRD、SEM、EDS和体外细胞研究以确定支架支持细胞粘附和维持活力的能力。支架支持细胞附着和维护时，观察了几天的时间，这表明他们的潜力，为未来的组织再生研究和应用。(C)2020作者（S）由爱思唯尔公司出版
Managing the vast amounts of eggshell and sea-shell waste that is produced globally each year is becoming very problematic as this material is simply disposed of in landfills. This waste results in odor production and microbial growth directly leading to environmental stresses. Transforming this waste into valuable biomaterials pose significant environmental and economic advantages as discarded waste have materials that can be utilized. The objective of this work was to develop inexpensive, bioactive hydroxyapatite-based scaffolds for tissue engineering applications by using an eco-friendly and sustainable approach. Engineering nano hydroxyapatite (EnHA) was derived from chicken eggshell and clam sea-shell waste by using an energy efficient microwave assisted wet chemical precipitation method. X-ray diffraction (XRD), Field emission scanning electron microscopy (FESEM), Transmission electron microscopy (TEM), and Energy dispersive spectroscopy (EDS) were used to analyze the biomaterials. Results show that the particles are highly crystalline hydroxyapatite (HA) with acicular shapes and within nanometer size ranges. The chemical compositions match exactly that of naturally occurring HA. The EnHA infused polymer scaffolds were fabricated using slurry-based solution 3D printing techniques. The slurry solutions composed of 70 wt% EnHA and 30 wt% Polycarpolactone (PCL). The XRD, SEM, EDS, and in vitro cell studies were conducted to determine the ability of the scaffolds to support cell adhesion and maintain viability. The scaffolds supported cellular attachment and maintenance when observed over a period of several days, suggesting their potential for future tissue regeneration research and applications. (C) 2020 The Author(s). Published by Elsevier B.V.