SURVIVAL OF MOUSE EMBRYOS AFTER FREEZING AND THAWING
SURVIVAL OF MOUSE EMBRYOS AFTER FREEZING AND THAWING
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DOI：
10.1038/233125a0
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发表时间：
1971-01-01
期刊：
影响因子：
64.8
通讯作者：
WHITTINGHAM, DG
中科院分区：
文献类型：
--
作者：
WHITTINGHAM, DG
与精子和其他组织细胞在零度以下的成功保存相比，保存冷冻哺乳动物卵子的尝试令人失望（参考文献中综述）。1和2），尽管少数妊娠是由于将先前冷冻的受精兔卵3和未受精的小鼠卵4转移给受体养母而导致的。我现在想描述一种在使胚胎经受零度以下的温度后获得高比例的可存活胚胎的技术。
COMPARED with the successful storage of spermatozoa and other tissue cells at sub-zero temperatures, attempts to preserve frozen mammalian eggs have been disappointing (reviewed in refs. 1 and 2), although a few pregnancies have resulted from the transfer of previously frozen fertilized rabbit eggs3and unfertilized mouse eggs4to recipient foster mothers. I now wish to describe a technique for obtaining a high proportion of viable embryos after subjecting them to sub-zero temperatures.