Anti-inflammatory, antibacterial, antioxidative bioactive glass-based nanofibrous dressing enables scarless wound healing
Anti-inflammatory, antibacterial, antioxidative bioactive glass-based nanofibrous dressing enables scarless wound healing
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DOI：
10.1016/j.smaim.2023.01.001
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发表时间：
2023-01
影响因子：
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通讯作者：
Zhengchao Yuan;Lixiang Zhang;Shichao Jiang;Muhammad Shafiq;Youjun Cai;Yujie Chen;Jiahui Song
中科院分区：
文献类型：
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作者：
Zhengchao Yuan;Lixiang Zhang;Shichao Jiang;Muhammad Shafiq;Youjun Cai;Yujie Chen;Jiahui Song
疤痕组织的过度形成以及细菌感染、出血和氧化性伤口微环境对患者的生理和心理造成了不利影响，这就需要创新的抗炎、抗菌、抗氧化多功能伤口敷料的出现。本研究的总体目标是利用生物活性玻璃（BG）和天然抗菌成分“牛至精油（OEO）”，为基于聚（l -丙交酯-羟基乙酸酯）/明胶（PLGA/Gel）的纳米纤维敷料提供多功能，用于切除伤口管理。我们进行了一系列的结构、形态和释放研究，并描绘了这些生物活性敷料在体外的血管生成、止血、抗菌和抗氧化特性，这些特性共同揭示了BG和OEO在快速止血、改善趋化反应、减少细菌定植和抗炎反应方面的有益作用。令人印象深刻的是，在多种损伤模型中，包括大鼠截尾模型、耳动脉损伤模型和兔肝损伤模型，我们报道了bg介导的快速止血。此外，体外活性部分凝血活素测定（APTT）显示，含有BG的敷料具有改善血液相容性和抑制凝血的作用。此外，将这些纳米纤维敷料移植到大鼠全层切除伤口模型中，显示出明显的组织再生，血管、腺体和毛囊数量增加，再上皮化，炎症反应减弱，纤维化组织形成减少。综合我们的方法，同时利用经济的BG和OEO，使组织修复的纳米纤维敷料具有多功能，这可能对伤口愈合以及其他生物相关学科有很大的希望。
Excessive scar tissue formation along with bacterial infection, hemorrhage, and oxidative wound microenvironment pose adverse physiological and psychological effects on patients, which necessitate the advent of innovative anti-inflammatory, anti-bacterial, and anti-oxidative multifunctional wound dressings. The overarching objective of this study was to exploit bioactive glass (BG) and a natural anti-bacterial component namely “oregano essential oil (OEO)” to impart multifunctionality to poly(L-lactide-co-glycolide)/Gelatin (PLGA/Gel)-based nanofibrous dressings for excisional wound management. We performed a series of structural, morphological, and release studies as well as delineated angiogenic, hemostatic, anti-bacterial, and anti-oxidative properties of these bioactive dressingsin vitro, which altogether revealed the beneficial effects of BG and OEO in terms of rapid hemostasis, improved chemotactic response, diminished bacterial colonization, and anti-inflammatory response. Impressively, in multiple injury models, including a rat tail-amputation model, an ear artery injury model, and a liver trauma model in rabbitin vivo, we reported BG-mediated rapid hemostasis. Moreover, dressings containing BG showed improved hemocompatibility and suppressed coagulation as revealed by activated partial thromboplastin assay (APTT)in vitro. In addition, the transplantation of these nanofibrous dressings in a full-thickness excisional wound model in rats showed significant tissue regeneration as evidenced by the more number of blood vessels, glands, and hair follicles, re-epithelialization, diminished inflammatory response, and less fibrotic tissue formation. Taken together our approach of simultaneously harnessing economical BG and OEO to enable multifunctionality to nanofibrous dressings for tissue repair may hold great promise for wound healing as well as other bio-related disciplines.