Proteome Analysis of Bronchoalveolar Lavage Fluid in Chronic Hypersensitivity Pneumonitis
Proteome Analysis of Bronchoalveolar Lavage Fluid in Chronic Hypersensitivity Pneumonitis
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DOI：
10.2332/allergolint.11-oa-0315
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发表时间：
2012-03-01
影响因子：
6.8
通讯作者：
Inase, Naohiko
中科院分区：
文献类型：
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作者：
Okamoto, Tsukasa;Miyazaki, Yasunari;Inase, Naohiko
背景：过敏性肺炎（HP）是一种由吸入多种抗原引起的免疫介导的肺部疾病。在病理学上，慢性HP倾向于显示普通间质性肺炎（UIP）和纤维化非特异性间质性肺炎（fNSIP）模式。方法：采用双向凝胶电泳和质谱技术对7例UIP型和4例fNSIP型患者的支气管肺泡灌洗液（BALF）蛋白质组进行分析，以评价UIP型和fNSIP型患者BALF蛋白质组的差异。通过单独比较每个BALF样本，我们发现表面活性蛋白A（SP-A）、免疫球蛋白重链α、α-2热休克糖蛋白、触珠蛋白β、免疫球蛋白J链在UIP型患者中的表达显著高于fNSIP型患者。与此相反，谷胱甘肽S-转移酶，维生素D-结合蛋白，β-肌动蛋白的蛋白水平在fNSIP模式的患者中显着高于UIP模式的患者。为了证实BALF蛋白质组中SP-A的结果，我们在更大的组中进行了酶联免疫吸附试验。UIP型患者BALF中SP-A浓度显著高于fNSIP型患者（2.331 +/- 1.656 μ g/ml vs. 1.319 +/- 1.916 μ g/ml，p = 0.034）。我们确定了几种蛋白质，可能在慢性HP的UIP和fNSIP模式之间的病理差异的发展中发挥作用。
Background: Hypersensitivity pneumonitis (HP) is an immune-mediated lung disease induced by inhalation of numerous antigens. Pathologically, chronic HP tends to show usual interstitial pneumonia (UIP) and fibrotic nonspecific interstitial pneumonia (fNSIP) patterns. Patients with UIP pattern present insidious onset and a risk for acute exacerbations.Methods: To evaluate the proteomic differences of bronchoalveolar lavage fluid (BALF) between UIP and fNSIP patterns, BALF from seven patients with UIP pattern and four patients with fNSIP pattern was examined using two-dimensional gel electrophoresis and mass spectrometry.Results: By individually comparing each BALF sample, we found that the protein levels of surfactant protein A (SP-A), immunoglobulin heavy chain alpha, alpha-2 heat shock glycoprotein, haptoglobin beta, and immunoglobulin J chain were significantly higher in the patients with UIP pattern than those in the patients with fNSIP pattern. In contrast, the protein levels of glutathione s-transferase, vitamin D-binding protein, and beta-actin were significantly higher in the patients with fNSIP pattern than those in the patients with UIP pattern. To confirm the results of SP-A in the BALF proteome, we performed enzyme-linked immunosorbent assay in a larger group. The concentrations of SP-A in BALF from the patients with UIP pattern were significantly higher than those from the patients with fNSIP pattern (2.331 +/- 1.656 mu g/ml vs. 1.319 +/- 1.916 mu g/ml, p = 0.034).Conclusions: We identified several proteins that may play roles in the development of pathological differences between UIP and fNSIP patterns of chronic HP.