Genome-wide comparative analysis of the SHSP, HSP60/10 and HSP90 genes reveals differential heat stress responses in estivation of the sea cucumber Apostichopus japonicus
Genome-wide comparative analysis of the SHSP, HSP60/10 and HSP90 genes reveals differential heat stress responses in estivation of the sea cucumber Apostichopus japonicus
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SHSP、HSP60/10 和 HSP90 基因的全基因组比较分析揭示了刺参夏眠时的不同热应激反应
DOI：
10.1111/are.13986
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发表时间：
2019
影响因子：
2
通讯作者：
Chongbo He
中科院分区：
文献类型：
--
作者：
Lei Gao;Zihao Yuan;Zhen Ma;Zhao Li;Simeng Yu;Yunfeng Li;Chongbo He
小分子热休克蛋白（Small HSP，SHSP）、热休克蛋白60/10和热休克蛋白90是热休克蛋白的一个亚类，在细胞保护、管家和凋亡调控等方面发挥重要作用。这些蛋白质总是参与对应激刺激的反应，特别是热应激。本研究从仿刺参（Apostichopus）的转录组和基因组中鉴定了10个SSPs、2个HSP 60/10和3个HSP 90，并对其进行了系统分析。系统发育分析和共线分析结果表明，虽然AjSHSPs进化程度较低，没有占据特定的结构域，但它们在进化过程中发生了温和的扩增，使海参成为本研究所评估的无脊椎动物中具有最多SHSPs的物种。ISHSPs类中五个拷贝的表达（AjHSP 24.3，AjHSP 25.5，AjHSP 25.6，AjHSP 26.1和AjHSP 29.8）在成虫夏眠期的表达量最大，而在幼龄个体中表达量差异较大，表明它们在对夏眠的反应中起着关键作用，并且成年和幼年个体在对慢性热休克的反应中使用了不同的策略。10和AjHSP 90被发现相对温和地调节，并被认为在管家中发挥额外的作用。这些结果为深入研究SHSP、HSP 60/10和HSP 90在海洋无脊椎动物夏眠中的作用提供了一个新的思路。
Small HSP (SHSP), HSP60/10 and HSP90 are subgroups of heat shock proteins and play important roles in cytoprotective, housekeeping and apoptosis regulatory functions. These proteins are always involved in the response to stressful stimuli, especially heat stress. In this study, 10SHSPs, twoHSP60/10sand threeHSP90swere identified and systematically characterized from the transcriptome and genome of the sea cucumberApostichopus japonicus. According to the results of phylogenetic and syntenic analyses, although theAjSHSPswere less evolved in that they did not occupy the specific domain, there was a mild expansion ofAjSHSPsthat made the sea cucumber the species that had the mostSHSPsamong the invertebrate species that were evaluated in this study. The expression of five copies in Class ISHSPs(AjHSP24.3,AjHSP25.5,AjHSP25.6,AjHSP26.1andAjHSP29.8) tended to be most regulated in estivation in adult individuals, while they exhibited differential patterns in juvenile individuals, suggesting their critical roles in the response to estivation and that different strategies were used in the response to chronic heat shock between adult and juvenile individuals.AjHSP60/10sandAjHSP90swere found to be relatively mildly regulated and were suggested to play additional roles in housekeeping. Together, these findings may provide an early insight into the involvement ofSHSPs,HSP60/10sandHSP90sin estivation of marine invertebrate.