Macrococcus canis sp nov., a skin bacterium associated with infections in dogs
Macrococcus canis sp nov., a skin bacterium associated with infections in dogs
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DOI：
10.1099/ijsem.0.001673
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发表时间：
2017-03-01
影响因子：
2.8
通讯作者：
Perreten, Vincent
中科院分区：
文献类型：
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作者：
Brawand, Stefanie Gobeli;Cotting, Kerstin;Perreten, Vincent
革兰氏染色阳性球菌分离自健康犬皮肤的其他部位以及犬的感染部位。16 S rRNA基因序列测定结果表明，与解酪巨球菌（Macrococcus caseolyticus）亲缘关系最近，同源性为99.7%，而与M.新菌株与caseolyticus的cpn 60、dnaJ、rpoB和sodA部分基因的同源性分别为90.8 ~ 93.5%。新菌株还表现出与M.大多数菌株在羊血琼脂上显示出明显的溶血，而M.溶酪菌没有任何溶血活性。他们产生了不同的基质辅助激光解吸/电离飞行时间（MALDI-TOF）MS光谱曲线相比，其他物种的属Macrococcus。KM 45013（T）株与M的模式株的DNA同源性仅为53.7%。这表明它们不属于同一物种。菌株KM 45013（T）的DNA G+C含量为36.9mol%。最丰富的脂肪酸是C-14：0、C-18（：）（3）omega 6c（6、9、12）和C-16：0。MK-6为KM 45013（T）的甲萘醌型。细胞壁结构分析表明，其肽聚糖类型为A3 a L-Lys-Gly（2）-L-Ser。根据基因型和化学分类学特征，我们建议将这些菌株分类为巨球菌属的一个新种，并建议将其命名为犬巨球菌（Macrococcuscanissp. nov.）。模式菌株是KM 45013（T）（=DSM 101690（T）=CCOS 969（T）=CCUG 68920（T））。
Gram-stain-positive cocci were isolated from miscellaneous sites of the skin of healthy dogs as well as from infection sites in dogs. The closest relative by sequencing of the 16S rRNA gene was Macrococcus caseolyticus with 99.7% sequence identity, but compared with M. caseolyticus, the novel strains shared only 90.8 to 93.5% DNA sequence identity with cpn60, dnaJ, rpoB and sodA partial genes, respectively. The novel strains also exhibited differential phenotypic characteristics from M. caseolyticus, and the majority displayed a visible haemolysis on sheep blood agar, while M. caseolyticus did not have any haemolytic activity. They generated different matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) MS spectral profiles compared with the other species of the genus Macrococcus. Furthermore, strain KM 45013(T) shared only 53.7% DNA-DNA relatedness with the type strain of M. caseolyticus, confirming that they do not belong to the same species. The DNA G+C content of strain KM 45013(T) was 36.9 mol%. The most abundant fatty acids were C-14 : 0, C-18 (:) (3)omega 6c (6, 9, 12) and C-16 : 0 n alcohol. MK-6 was the menaquinone type of KM 45013(T). Cell-wall structure analysis revealed that the peptidoglycan type was A3a L-Lys-Gly(2)-L-Ser. Based on genotypic and chemotaxonomic characteristics, we propose to classify these strains within a novel species of the genus Macrococcus for which the name Macrococcus canis sp. nov. is proposed. The type strain is KM 45013(T) (=DSM 101690(T) =CCOS 969(T) =CCUG 68920(T)).