D-Mannose Enhanced Immunomodulation of Periodontal Ligament Stem Cells via Inhibiting IL-6 Secretion
D-Mannose Enhanced Immunomodulation of Periodontal Ligament Stem Cells via Inhibiting IL-6 Secretion
复制标题
D-甘露糖通过抑制 IL-6 分泌增强牙周膜干细胞的免疫调节
DOI：
10.1155/2018/7168231
复制
发表时间：
2018-01-01
影响因子：
4.3
通讯作者：
Bai, Yuxing
中科院分区：
文献类型：
--
作者：
Guo, Lijia;Hou, Yanan;Bai, Yuxing
牙周膜干细胞介导的牙周组织再生是近年来提出的一种治疗牙槽骨和牙周膜缺损的新方法。有报道称，自体和异体牙周膜干细胞均可重建受损牙周组织，但再生效果并不一致。应进一步考虑改善PDLSC性能的有效方法。在这项研究中，我们研究了D-甘露糖是否会影响hPDLSCs的免疫调节特性。经D-甘露糖预处理后，hPDLSCs可抑制T细胞增殖，影响T细胞向Treg细胞的分化。我们发现在D-甘露糖预处理的hPDLSC中可以检测到较少的IL-6。在D-甘露糖预处理组中，如果可以检测到增加的IL-6水平，则诱导的Treg细胞数量将减少。我们的数据揭示了D-甘露糖调节PDLSC免疫调节功能的一种以前未被认识的功能，并且IL-6可能在此过程中发挥关键作用。实验结果为促进牙周组织再生提供了一种可行的方法。
Periodontal ligament stem cell- (PDLSC-) mediated periodontal tissue regeneration has recently been proposed for the new therapeutic method to regenerate lost alveolar bone and periodontal ligament. It was reported that both autogenic and allogeneic PDLSCs could reconstruct damaged periodontal tissues but the regeneration effects were not consistent. The effective methods to improve the properties of PDLSCs should be further considered. In this study, we investigated if D-mannose could affect the immunomodulatory properties of hPDLSCs. After being pretreated with D-mannose, hPDLSCs could inhibit T cell proliferation and affect T cell differentiation into Treg cells. We found that less IL-6 could be detected in D-mannose-pretreated hPDLSCs. In the D-mannose pretreatment group, induced Treg cell number would decrease if increased IL-6 levels could be detected. Our data uncovered a previously unrecognized function of D-mannose to regulate the immunomodulatory function of PDLSCs and that IL-6 might play a key role in this process. The results provided a property method to improve PDLSC-based periodontal regeneration.