Transforming growth factor-β-induced stimulation of formation of collagen fiber network and anti-fibrotic effect of taurine in an in vitro model of hepatic fibrosis
Transforming growth factor-β-induced stimulation of formation of collagen fiber network and anti-fibrotic effect of taurine in an in vitro model of hepatic fibrosis
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DOI：
10.1016/j.hepres.2004.04.006
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发表时间：
2004-09-01
影响因子：
4.2
通讯作者：
Tsubouchi, H
中科院分区：
文献类型：
--
作者：
Kato, J;Ido, A;Tsubouchi, H
The cell strain M, which was established from normal rat liver cells, is characterized by the active formation of a collagen fiber network. In this study, we investigated the characterization of M cells and evaluated the anti-fibrogenic effects of taurine using this culture system. M cells expressed cytokeratin (CK)8, CK18, vimentin, and alpha-smooth muscle actin, whereas expression of CK-19 or desmin was not detected. Also, M cells expressed transforming growth factor (TGF)-beta1, -beta2, and TGF-beta type I and II receptors, and treatment with TGF-beta1 (1 ng/ml) for 6 days markedly stimulated the formation of a collagen fiber network and expression of procollagen alpha1 (I) mRNA. When M cells were treated with various concentrations of taurine (10-50 mM), network formation and procollagen alpha1 (I) expression were significantly suppressed in a dose dependent manner. Additionally, even in the presence of TGF-beta1, taurine treatment effectively reduced the formation of a collagen fiber network. These results suggest that M cells exhibit features of not only hepatocytes but also myofibroblasts, and TGF-beta1 plays an important role in the formation of collagen fiber networks in this culture system. Additionally, this M cell culture system is appropriate for use as an in vitro model of hepatic fibrosis in the evaluation of the anti-fibrogenic effects of various agents. (C) 2004 Elsevier B.V. All rights reserved.
