The complete genome sequence of Mycobacterium bovis
The complete genome sequence of Mycobacterium bovis
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DOI：
10.1073/pnas.1130426100
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发表时间：
2003-06-24
影响因子：
11.1
通讯作者：
Hewinson, RG
中科院分区：
文献类型：
--
作者：
Garnier, T;Eiglmeier, K;Hewinson, RG
牛分枝杆菌是一系列动物物种和人的结核病的病原体，全世界每年给农业造成30亿美元的损失。由牛结核杆菌引起的人类结核病负担在很大程度上仍然未知。M.牛也是M.牛卡介苗是最广泛使用的人用疫苗。在此我们描述了M.牛AF 2122/97的基因组及其与结核分枝杆菌和麻风分枝杆菌基因组的比较。引人注目的是，M. bovis与M.结核病，但基因信息的缺失导致基因组大小缩小。与M比较麻风揭示了许多常见的基因丢失，表明功能冗余的去除。细胞壁组分和分泌蛋白显示出最大的变化，表明它们在宿主-芽孢杆菌相互作用或免疫逃避中的潜在作用。此外，M没有独特的基因。这表明，差异基因表达可能是人和牛杆菌宿主嗜性的关键。因此，基因组序列提供了重要的洞察力的进化，主机偏好，和病理生物学的M。牛。
Mycobacterium bovis is the causative agent of tuberculosis in a range of animal species and man, with worldwide annual losses to agriculture of $3 billion. The human burden of tuberculosis caused by the bovine tubercle bacillus is still largely unknown. M. bovis was also the progenitor for the M. bovis bacillus Calmette-Guerin vaccine strain, the most widely used human vaccine. Here we describe the 4,345,492-bp genome sequence of M. bovis AF2122/97 and its comparison with the genomes of Mycobacterium tuberculosis and Mycobacterium leprae. Strikingly, the genome sequence of M. bovis is >99.95% identical to that of M. tuberculosis, but deletion of genetic information has led to a reduced genome size. Comparison with M. leprae reveals a number of common gene losses, suggesting the removal of functional redundancy. Cell wall components and secreted proteins show the greatest variation, indicating their potential role in host-bacillus interactions or immune evasion. Furthermore, there are no genes unique to M. bovis, implying that differential gene expression may be the key to the host tropisms of human and bovine bacilli. The genome sequence therefore offers major insight on the evolution, host preference, and pathobiology of M. bovis.