S100A9-positive granulocytes and monocytes in lipopolysaccharide-induced anterior ocular inflammation
S100A9-positive granulocytes and monocytes in lipopolysaccharide-induced anterior ocular inflammation
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DOI：
10.1016/j.exer.2006.09.016
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发表时间：
2007-02-01
影响因子：
3.4
通讯作者：
Hayasaka, Seiji
中科院分区：
文献类型：
--
作者：
Chi, Zai-Long;Hayasaka, Yoriko;Hayasaka, Seiji
S100 A9是在浸润性粒细胞和单核细胞中表达的促炎蛋白。我们确定了S100 A9在Wistar大鼠内毒素（LPS）诱导的葡萄膜炎（EIU）和角膜炎中的作用。与LPS组相比，抗S100 A9抗体在18-36 h降低了部分临床评分、蛋白和房水中的细胞。S100A.97阳性细胞在2448 h时在虹膜睫状体（ICB）和角膜表达。LPS注射后18-48 h，ICB中活化的caspase-3（与细胞凋亡相关）和S100 A9共表达。ICB中ED 2阳性细胞不表达S100 A9。地塞米松（DEX）可使LPS诱导的循环血白细胞S100 A9 mRNA和蛋白水平升高，而ICB中S100 A9 mRNA和蛋白水平降低。BAY 11-7085（一种I-KB磷酸化抑制剂）在LPS注射后分别抑制白细胞（43.5%）和ICB（68.5%）中的S100 A9 mRNA。S100 A9阳性的粒细胞和单核/巨噬细胞可能在EIU和角膜炎的晚期发挥作用，DEX可能抑制了S100 A9阳性的粒细胞和单核细胞从血液向血管外组织的迁移，核因子（NF）-κ B通路可能参与了S100 A9的表达。S100 OA 9在EIU晚期可能参与炎症细胞的清除。(c)2006爱思唯尔有限公司保留所有权利。
S100A9 is a pro-inflammatory protein expressed in infiltrating granulocytes and monocytes. We determined role of S100A9 in endotoxin (LPS)-induced uveitis (EIU) and keratitis in Wistar rats. Anti-S100A9 antibody decreased partially clinical scores, protein, and cells in the aqueous humor at 18-36 h, compared with the LPS group. S100A.97 positive cells were expressed in the iris-ciliary body (ICB) and cornea at 2448 h. Activated caspase-3 (related to apoptosis) and S100A9 co-expressed in ICB at 18-48 h after LPS injection. S100A9 was not expressed in ED2-positive cells in ICB. Dexamethasone (DEX) increased S100A9 mRNA and protein levels in the circulating blood leukocytes, but reduced S100A9 mRNA and protein levels in ICB after LPS injection. BAY 11-7085 (an inhibitor of I-KB phosphorylation) suppressed S100A9 mRNA in leukocytes (43.5%) and ICB (68.5%), respectively, after LPS injection. It is possible that S100A9-positive granulocytes and monocyte/ macrophages may play a role in the late phase of EIU and keratitis that DEX may inhibit the migration of S100A9-positive granulocytes and monocytes from the blood into the extravascular tissues, and that nuclear factor (NF)-KB pathway may be involved in S10OA9 expression. S100OA9 could play a role in the clearance of inflammatory cells at the late phase of EIU. (c) 2006 Elsevier Ltd. All rights reserved.