Prominent Actin Fiber Arrays in Drosophila Tendon Cells Represent Architectural Elements Different from Stress Fibers
Prominent Actin Fiber Arrays in Drosophila Tendon Cells Represent Architectural Elements Different from Stress Fibers
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DOI：
10.1091/mbc.e08-02-0182
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发表时间：
2008-10-01
影响因子：
3.3
通讯作者：
Prokop, Andreas
中科院分区：
文献类型：
--
作者：
Alves-Silva, Juliana;Hahn, Ines;Prokop, Andreas
腱细胞是昆虫表皮的特化细胞，通过显著的微管阵列将基部附着的肌尖连接到其顶端表面的角质层。果蝇的腱细胞已成为解决细胞和发育生物学相关问题的有用的遗传模型系统。在这里，我们使用光，共聚焦和电子显微镜，提出了一个精致的模型肌腱细胞的亚细胞组织。我们发现，突出的F-肌动蛋白阵列存在于肌腱细胞，完全重叠的微管阵列，和II型肌球蛋白积累在同一地区。肌腱细胞中的F-肌动蛋白阵列似乎代表了一种新的肌动蛋白结构，与应力纤维明显不同。它们对F-肌动蛋白去稳定化药物、肌球蛋白阻滞剂的应用以及整联蛋白、Rho 1或机械力的丧失具有高度抗性。它们似乎代表了肌腱细胞的重要结构元素，因为它们即使在肌腱细胞的微管阵列功能障碍时也保持顶面和底面之间的连接。这里和其他地方报道的肌腱细胞的特征让人想起脊椎动物内耳支持细胞的结构和分子特征，它们可能具有潜在的翻译价值。
Tendon cells are specialized cells of the insect epidermis that connect basally attached muscle tips to the cuticle on their apical surface via prominent arrays of microtubules. Tendon cells of Drosophila have become a useful genetic model system to address questions with relevance to cell and developmental biology. Here, we use light, confocal, and electron microscopy to present a refined model of the subcellular organization of tendon cells. We show that prominent arrays of F-actin exist in tendon cells that fully overlap with the microtubule arrays, and that type II myosin accumulates in the same area. The F-actin arrays in tendon cells seem to represent a new kind of actin structure, clearly distinct from stress fibers. They are highly resistant to F-actin-destabilizing drugs, to the application of myosin blockers, and to loss of integrin, Rho1, or mechanical force. They seem to represent an important architectural element of tendon cells, because they maintain a connection between apical and basal surfaces even when microtubule arrays of tendon cells are dysfunctional. Features reported here and elsewhere for tendon cells are reminiscent of the structural and molecular features of support cells in the inner ear of vertebrates, and they might have potential translational value.