Robust, universal biomarker assay to detect senescent cells in biological specimens.
Robust, universal biomarker assay to detect senescent cells in biological specimens.
复制标题
用于检测生物样本中衰老细胞的稳健、通用的生物标志物测定。
DOI：
10.1111/acel.12545
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发表时间：
2017-02
期刊：
影响因子：
7.8
通讯作者：
Gorgoulis VG
中科院分区：
文献类型：
--
作者：
Evangelou K;Lougiakis N;Rizou SV;Kotsinas A;Kletsas D;Muñoz-Espín D;Kastrinakis NG;Pouli N;Marakos P;Townsend P;Serrano M;Bartek J;Gorgoulis VG
Cellular senescence contributes to organismal development, aging, and diverse pathologies, yet available assays to detect senescent cells remain unsatisfactory. Here, we designed and synthesized a lipophilic, biotin‐linked Sudan Black B (SBB) analogue suitable for sensitive and specific, antibody‐enhanced detection of lipofuscin‐containing senescent cells in any biological material. This new hybrid histo‐/immunochemical method is easy to perform, reliable, and universally applicable to assess senescence in biomedicine, from cancer research to gerontology.
