Bronchial epithelial cell B7-1 and B7-2 mRNA expression after lung transplantation:: a role in allograft rejection?
Bronchial epithelial cell B7-1 and B7-2 mRNA expression after lung transplantation:: a role in allograft rejection?
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DOI：
10.1183/09031936.02.00268102
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发表时间：
2002-07-01
影响因子：
24.3
通讯作者：
Vogelmeier, C
中科院分区：
文献类型：
--
作者：
Elssner, A;Jaumann, F;Vogelmeier, C
闭塞性细支气管炎通常被解释为慢性排斥反应，累及支气管和细支气管上皮。支气管上皮细胞（BEC）上主要组织相容性复合物（MHC）II的上调被假设为是支气管定向排斥反应的重要触发因素。最近，发现抗原呈递细胞上共刺激分子B7-1（CD 80）和B7-2（CD 86）的额外表达在移植排斥中T淋巴细胞的活化中起重要作用。BEC表达这些分子的作用尚不清楚，通过支气管刷和支气管肺泡灌洗液（BALF）细胞从肺移植受体获得的BEC进行了研究，并通过半定量逆转录-聚合酶链反应评估B7-1和B7-2的信使核糖核酸（mRNA）表达。在肺移植后的前3个月内，B7-1/甘油醛-3-磷酸脱氢酶（GAPDH）mRNA的比值在BEC中显著升高。有趣的是，在一小群闭塞性细支气管炎综合征患者中，B7-1/GAPDH和B7-2/GAPDH比值在BEC中显著升高，而在BALF细胞中没有发现差异。
Obliterative bronchiolitis is commonly interpreted as chronic rejection and involves the bronchial and bronchiolar epithelium. Upregulation of major histocompatibility complex (MHC) II on bronchial epithelial cells (BEC) had been hypothesised to be an important trigger of a bronchus directed rejection response. More recently, the additional expression of the costimulatory molecules B7-1 (CD80) and B7-2 (CD86) on antigen presenting cells were found to play, an important role in the activation of T-lymphocytes in transplant rejection. The role of the expression of these molecules by BEC is unclear.BEC obtained by bronchial brushing and bronchoalveolar lavage fluid (BALF) cells from lung transplant recipients were studied and evaluated for messenger ribonucleic acid (mRNA) expression of B7-1 and B7-2 by semi-quantitative reverse transcriptase-polymerase chain reaction. Significantly elevated B7-1/glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA ratios were found in BEC from patients examined during the first 3 months after lung transplantation. Interestingly, in a small group of patients with bronchiolitis obliterans syndrome the B7-1/GAPDH and B7-2/GAPDH ratios were significantly elevated for BEC, whereas no differences were found for the BALF cells.In summary, B7 messenger ribonucleic acid expression by bronchial epithelial cells may play a role in (chronic) lung allograft rejection.