Microbiota of the Small Intestine Is Selectively Engulfed by Phagocytes of the Lamina Propria and Peyer's Patches.
Microbiota of the Small Intestine Is Selectively Engulfed by Phagocytes of the Lamina Propria and Peyer's Patches.
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DOI：
10.1371/journal.pone.0163607
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Matsumoto S
中科院分区：
文献类型：
--
作者：
Morikawa M;Tsujibe S;Kiyoshima-Shibata J;Watanabe Y;Kato-Nagaoka N;Shida K;Matsumoto S
分布在小肠粘膜中的树突状细胞和巨噬细胞等吞噬细胞通过对肠腔微生物群进行采样，在维持粘膜稳态方面发挥着至关重要的作用。然而，有关稳定状态下微生物吸收的信息有限。我们研究了被小肠固有层 (SILP) 和派尔氏集结 (PP) 中特定亚群的吞噬细胞吞噬的小鼠肠道微生物群的组成。对细菌16S rRNA基因扩增子序列的分析表明：1）SILP中的所有吞噬细胞亚群主要吞噬乳酸杆菌（小肠中最丰富的微生物），而PP中的CD11bhi和CD11bhiCD11chi细胞亚群主要吞噬分段丝状细菌（据报道粘附于肠上皮细胞的啮齿类动物的本土细菌）； 2) 在被 SILP 细胞亚群吞噬的乳杆菌种中，L. murinus 比 L. taiwanensis 被吞噬的频率更高，尽管这两种乳杆菌种在生理条件下在小肠中含量丰富。这些结果表明，小肠微生物群选择性地被稳定状态下位于邻近肠粘膜的吞噬细胞吞噬。这些观察结果可能有助于了解吞噬细胞在小肠粘膜免疫监视中的关键作用。
Phagocytes such as dendritic cells and macrophages, which are distributed in the small intestinal mucosa, play a crucial role in maintaining mucosal homeostasis by sampling the luminal gut microbiota. However, there is limited information regarding microbial uptake in a steady state. We investigated the composition of murine gut microbiota that is engulfed by phagocytes of specific subsets in the small intestinal lamina propria (SILP) and Peyer’s patches (PP). Analysis of bacterial 16S rRNA gene amplicon sequences revealed that: 1) all the phagocyte subsets in the SILP primarily engulfed Lactobacillus (the most abundant microbe in the small intestine), whereas CD11bhi and CD11bhiCD11chi cell subsets in PP mostly engulfed segmented filamentous bacteria (indigenous bacteria in rodents that are reported to adhere to intestinal epithelial cells); and 2) among the Lactobacillus species engulfed by the SILP cell subsets, L. murinus was engulfed more frequently than L. taiwanensis, although both these Lactobacillus species were abundant in the small intestine under physiological conditions. These results suggest that small intestinal microbiota is selectively engulfed by phagocytes that localize in the adjacent intestinal mucosa in a steady state. These observations may provide insight into the crucial role of phagocytes in immune surveillance of the small intestinal mucosa.
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影响因子：
64.5
作者：
Ivanov, Ivaylo I.;McKenzie, Brent S.;Littman, Dan R.
通讯作者：
Littman, Dan R.
DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
32.4
作者：
Bunker JJ;Flynn TM;Koval JC;Shaw DG;Meisel M;McDonald BD;Ishizuka IE;Dent AL;Wilson PC;Jabri B;Antonopoulos DA;Bendelac A
通讯作者：
Bendelac A
影响因子：
2.2
作者：
Takada, T;Matsumoto, K;Nomoto, K
通讯作者：
Nomoto, K
DOI：
10.1073/pnas.1000080107
发表时间：
2011-03-15
影响因子：
11.1
作者：
Caporaso, J. Gregory;Lauber, Christian L.;Knight, Rob
通讯作者：
Knight, Rob